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Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells
BACKGROUND: RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates. METHODS: To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following n...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567309/ https://www.ncbi.nlm.nih.gov/pubmed/18817543 http://dx.doi.org/10.1186/1749-7221-3-19 |
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author | Fuentes, Erick O Leemhuis, Jost Stark, G Björn Lang, Eva M |
author_facet | Fuentes, Erick O Leemhuis, Jost Stark, G Björn Lang, Eva M |
author_sort | Fuentes, Erick O |
collection | PubMed |
description | BACKGROUND: RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates. METHODS: To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate. |
format | Text |
id | pubmed-2567309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-25673092008-10-15 Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells Fuentes, Erick O Leemhuis, Jost Stark, G Björn Lang, Eva M J Brachial Plex Peripher Nerve Inj Research Article BACKGROUND: RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates. METHODS: To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate. BioMed Central 2008-09-25 /pmc/articles/PMC2567309/ /pubmed/18817543 http://dx.doi.org/10.1186/1749-7221-3-19 Text en Copyright © 2008 Fuentes et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Fuentes, Erick O Leemhuis, Jost Stark, G Björn Lang, Eva M Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells |
title | Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells |
title_full | Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells |
title_fullStr | Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells |
title_full_unstemmed | Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells |
title_short | Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells |
title_sort | rho kinase inhibitors y27632 and h1152 augment neurite extension in the presence of cultured schwann cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567309/ https://www.ncbi.nlm.nih.gov/pubmed/18817543 http://dx.doi.org/10.1186/1749-7221-3-19 |
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