Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713

On the basis of high enzyme activity a newly isolated strain of L. mesenteroides CMG713 was selected for dextran production. For maximum yield of dextran, effects of various parameters such as pH, temperature, sucrose concentration and incubation period were studied. L. mesenteroides CMG713 produced...

Descripción completa

Detalles Bibliográficos
Autores principales: Sarwat, Farwa, Qader, Shah Ali Ul, Aman, Afsheen, Ahmed, Nuzhat
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2008
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567811/
https://www.ncbi.nlm.nih.gov/pubmed/18953402
_version_ 1782160014748680192
author Sarwat, Farwa
Qader, Shah Ali Ul
Aman, Afsheen
Ahmed, Nuzhat
author_facet Sarwat, Farwa
Qader, Shah Ali Ul
Aman, Afsheen
Ahmed, Nuzhat
author_sort Sarwat, Farwa
collection PubMed
description On the basis of high enzyme activity a newly isolated strain of L. mesenteroides CMG713 was selected for dextran production. For maximum yield of dextran, effects of various parameters such as pH, temperature, sucrose concentration and incubation period were studied. L. mesenteroides CMG713 produced maximum dextran after 20 hours of incubation at 30ºC with 15% sucrose at pH 7.0. The molecular mass distribution of dextran produced by this strain showed that its molecular mass was about 2.0 million Da. Dextran analysis by (13)C-NMR spectrometry showed no signals corresponding to any other linkages except α-(1→6) glycosidic linkage in the main chain, which has not been reported before. Physico-chemical properties of this unique dextran were also studied. These optimised conditions could be used for the commercial production of this unique high molecular weight dextran, which have significant industrial perspectives.
format Text
id pubmed-2567811
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-25678112008-10-24 Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713 Sarwat, Farwa Qader, Shah Ali Ul Aman, Afsheen Ahmed, Nuzhat Int J Biol Sci Research Paper On the basis of high enzyme activity a newly isolated strain of L. mesenteroides CMG713 was selected for dextran production. For maximum yield of dextran, effects of various parameters such as pH, temperature, sucrose concentration and incubation period were studied. L. mesenteroides CMG713 produced maximum dextran after 20 hours of incubation at 30ºC with 15% sucrose at pH 7.0. The molecular mass distribution of dextran produced by this strain showed that its molecular mass was about 2.0 million Da. Dextran analysis by (13)C-NMR spectrometry showed no signals corresponding to any other linkages except α-(1→6) glycosidic linkage in the main chain, which has not been reported before. Physico-chemical properties of this unique dextran were also studied. These optimised conditions could be used for the commercial production of this unique high molecular weight dextran, which have significant industrial perspectives. Ivyspring International Publisher 2008-10-15 /pmc/articles/PMC2567811/ /pubmed/18953402 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Sarwat, Farwa
Qader, Shah Ali Ul
Aman, Afsheen
Ahmed, Nuzhat
Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713
title Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713
title_full Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713
title_fullStr Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713
title_full_unstemmed Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713
title_short Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713
title_sort production & characterization of a unique dextran from an indigenous leuconostoc mesenteroides cmg713
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567811/
https://www.ncbi.nlm.nih.gov/pubmed/18953402
work_keys_str_mv AT sarwatfarwa productioncharacterizationofauniquedextranfromanindigenousleuconostocmesenteroidescmg713
AT qadershahaliul productioncharacterizationofauniquedextranfromanindigenousleuconostocmesenteroidescmg713
AT amanafsheen productioncharacterizationofauniquedextranfromanindigenousleuconostocmesenteroidescmg713
AT ahmednuzhat productioncharacterizationofauniquedextranfromanindigenousleuconostocmesenteroidescmg713

Ejemplares similares