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The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis

BACKGROUND: IkappaB kinases (IKKs) regulate the activity of Rel/NF-kappaB transcription factors by targeting their inhibitory partner proteins, IkappaBs, for degradation. The Drosophila genome encodes two members of the IKK family. Whereas the first is a kinase essential for activation of the NF-kap...

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Autores principales: Dubin-Bar, Dikla, Bitan, Amir, Bakhrat, Anna, Kaiden-Hasson, Rotem, Etzion, Sharon, Shaanan, Boaz, Abdu, Uri
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567969/
https://www.ncbi.nlm.nih.gov/pubmed/18796167
http://dx.doi.org/10.1186/1471-2121-9-51
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author Dubin-Bar, Dikla
Bitan, Amir
Bakhrat, Anna
Kaiden-Hasson, Rotem
Etzion, Sharon
Shaanan, Boaz
Abdu, Uri
author_facet Dubin-Bar, Dikla
Bitan, Amir
Bakhrat, Anna
Kaiden-Hasson, Rotem
Etzion, Sharon
Shaanan, Boaz
Abdu, Uri
author_sort Dubin-Bar, Dikla
collection PubMed
description BACKGROUND: IkappaB kinases (IKKs) regulate the activity of Rel/NF-kappaB transcription factors by targeting their inhibitory partner proteins, IkappaBs, for degradation. The Drosophila genome encodes two members of the IKK family. Whereas the first is a kinase essential for activation of the NF-kappaB pathway, the latter does not act as IkappaB kinase. Instead, recent findings indicate that Ik2 regulates F-actin assembly by mediating the function of nonapoptotic caspases via degradation of DIAP1. Also, it has been suggested that ik2 regulates interactions between the minus ends of the microtubules and the actin-rich cortex in the oocyte. Since spn-F mutants display oocyte defects similar to those of ik2 mutant, we decided to investigate whether Spn-F could be a direct regulatory target of Ik2. RESULTS: We found that Ik2 binds physically to Spn-F, biomolecular interaction analysis of Spn-F and Ik2 demonstrating that both proteins bind directly and form a complex. We showed that Ik2 phosphorylates Spn-F and demonstrated that this phosphorylation does not lead to Spn-F degradation. Ik2 is localized to the anterior ring of the oocyte and to punctate structures in the nurse cells together with Spn-F protein, and both proteins are mutually required for their localization. CONCLUSION: We conclude that Ik2 and Spn-F form a complex, which regulates cytoskeleton organization during Drosophila oogenesis and in which Spn-F is the direct regulatory target for Ik2. Interestingly, Ik2 in this complex does not function as a typical IKK in that it does not direct SpnF for degradation following phosphorylation.
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spelling pubmed-25679692008-10-16 The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis Dubin-Bar, Dikla Bitan, Amir Bakhrat, Anna Kaiden-Hasson, Rotem Etzion, Sharon Shaanan, Boaz Abdu, Uri BMC Cell Biol Research Article BACKGROUND: IkappaB kinases (IKKs) regulate the activity of Rel/NF-kappaB transcription factors by targeting their inhibitory partner proteins, IkappaBs, for degradation. The Drosophila genome encodes two members of the IKK family. Whereas the first is a kinase essential for activation of the NF-kappaB pathway, the latter does not act as IkappaB kinase. Instead, recent findings indicate that Ik2 regulates F-actin assembly by mediating the function of nonapoptotic caspases via degradation of DIAP1. Also, it has been suggested that ik2 regulates interactions between the minus ends of the microtubules and the actin-rich cortex in the oocyte. Since spn-F mutants display oocyte defects similar to those of ik2 mutant, we decided to investigate whether Spn-F could be a direct regulatory target of Ik2. RESULTS: We found that Ik2 binds physically to Spn-F, biomolecular interaction analysis of Spn-F and Ik2 demonstrating that both proteins bind directly and form a complex. We showed that Ik2 phosphorylates Spn-F and demonstrated that this phosphorylation does not lead to Spn-F degradation. Ik2 is localized to the anterior ring of the oocyte and to punctate structures in the nurse cells together with Spn-F protein, and both proteins are mutually required for their localization. CONCLUSION: We conclude that Ik2 and Spn-F form a complex, which regulates cytoskeleton organization during Drosophila oogenesis and in which Spn-F is the direct regulatory target for Ik2. Interestingly, Ik2 in this complex does not function as a typical IKK in that it does not direct SpnF for degradation following phosphorylation. BioMed Central 2008-09-17 /pmc/articles/PMC2567969/ /pubmed/18796167 http://dx.doi.org/10.1186/1471-2121-9-51 Text en Copyright © 2008 Dubin-Bar et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dubin-Bar, Dikla
Bitan, Amir
Bakhrat, Anna
Kaiden-Hasson, Rotem
Etzion, Sharon
Shaanan, Boaz
Abdu, Uri
The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis
title The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis
title_full The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis
title_fullStr The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis
title_full_unstemmed The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis
title_short The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis
title_sort drosophila ikk-related kinase (ik2) and spindle-f proteins are part of a complex that regulates cytoskeleton organization during oogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567969/
https://www.ncbi.nlm.nih.gov/pubmed/18796167
http://dx.doi.org/10.1186/1471-2121-9-51
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