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An amino acid at position 142 in nitrilase from Rhodococcus rhodochrous ATCC 33278 determines the substrate specificity for aliphatic and aromatic nitriles

Nitrilase from Rhodococcus rhodochrous ATCC 33278 hydrolyses both aliphatic and aromatic nitriles. Replacing Tyr-142 in the wild-type enzyme with the aromatic amino acid phenylalanine did not alter specificity for either substrate. However, the mutants containing non-polar aliphatic amino acids (ala...

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Detalles Bibliográficos
Autores principales:	Yeom, Soo-Jin, Kim, Hye-Jung, Lee, Jung-Kul, Kim, Dong-Eun, Oh, Deok-Kun
Formato:	Texto
Lenguaje:	English
Publicado:	Portland Press Ltd. 2008
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570083/ https://www.ncbi.nlm.nih.gov/pubmed/18412544 http://dx.doi.org/10.1042/BJ20080440

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