Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging

The Wnt/β-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. β-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper...

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Detalles Bibliográficos
Autor principal: Hannoush, Rami N.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570213/
https://www.ncbi.nlm.nih.gov/pubmed/18941539
http://dx.doi.org/10.1371/journal.pone.0003498
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author Hannoush, Rami N.
author_facet Hannoush, Rami N.
author_sort Hannoush, Rami N.
collection PubMed
description The Wnt/β-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. β-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular β-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of β-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of β-catenin.
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spelling pubmed-25702132008-10-22 Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging Hannoush, Rami N. PLoS One Research Article The Wnt/β-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. β-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular β-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of β-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of β-catenin. Public Library of Science 2008-10-22 /pmc/articles/PMC2570213/ /pubmed/18941539 http://dx.doi.org/10.1371/journal.pone.0003498 Text en Hannoush. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hannoush, Rami N.
Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging
title Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging
title_full Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging
title_fullStr Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging
title_full_unstemmed Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging
title_short Kinetics of Wnt-Driven β-Catenin Stabilization Revealed by Quantitative and Temporal Imaging
title_sort kinetics of wnt-driven β-catenin stabilization revealed by quantitative and temporal imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570213/
https://www.ncbi.nlm.nih.gov/pubmed/18941539
http://dx.doi.org/10.1371/journal.pone.0003498
work_keys_str_mv AT hannoushramin kineticsofwntdrivenbcateninstabilizationrevealedbyquantitativeandtemporalimaging

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