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Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults

BACKGROUND: Antibodies that impair Plasmodium falciparum merozoite invasion and intraerythrocytic development are one of several mechanisms that mediate naturally acquired immunity to malaria. Attempts to correlate anti-malaria antibodies with risk of infection and morbidity have yielded inconsisten...

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Autores principales: Dent, Arlene E., Bergmann-Leitner, Elke S., Wilson, Danny W., Tisch, Daniel J., Kimmel, Rhonda, Vulule, John, Sumba, Peter Odada, Beeson, James G., Angov, Evelina, Moormann, Ann M., Kazura, James W.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570335/
https://www.ncbi.nlm.nih.gov/pubmed/18958285
http://dx.doi.org/10.1371/journal.pone.0003557
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author Dent, Arlene E.
Bergmann-Leitner, Elke S.
Wilson, Danny W.
Tisch, Daniel J.
Kimmel, Rhonda
Vulule, John
Sumba, Peter Odada
Beeson, James G.
Angov, Evelina
Moormann, Ann M.
Kazura, James W.
author_facet Dent, Arlene E.
Bergmann-Leitner, Elke S.
Wilson, Danny W.
Tisch, Daniel J.
Kimmel, Rhonda
Vulule, John
Sumba, Peter Odada
Beeson, James G.
Angov, Evelina
Moormann, Ann M.
Kazura, James W.
author_sort Dent, Arlene E.
collection PubMed
description BACKGROUND: Antibodies that impair Plasmodium falciparum merozoite invasion and intraerythrocytic development are one of several mechanisms that mediate naturally acquired immunity to malaria. Attempts to correlate anti-malaria antibodies with risk of infection and morbidity have yielded inconsistent results. Growth inhibition assays (GIA) offer a convenient method to quantify functional antibody activity against blood stage malaria. METHODS: A treatment-time-to-infection study was conducted over 12-weeks in a malaria holoendemic area of Kenya. Plasma collected from healthy individuals (98 children and 99 adults) before artemether-lumefantrine treatment was tested by GIA in three separate laboratories. RESULTS: Median GIA levels varied with P. falciparum line (D10, 8.8%; 3D7, 34.9%; FVO, 51.4% inhibition). The magnitude of growth inhibition decreased with age in all P. falciparum lines tested with the highest median levels among children <4 years compared to adults (e.g. 3D7, 45.4% vs. 30.0% respectively, p = 0.0003). Time-to-infection measured by weekly blood smears was significantly associated with level of GIA controlling for age. Upper quartile inhibition activity was associated with less risk of infection compared to individuals with lower levels (e.g. 3D7, hazard ratio = 1.535, 95% CI = 1.012–2.329; p = 0.0438). Various GIA methodologies had little effect on measured parasite growth inhibition. CONCLUSION: Plasma antibody-mediated growth inhibition of blood stage P. falciparum decreases with age in residents of a malaria holoendemic area. Growth inhibition assay may be a useful surrogate of protection against infection when outcome is controlled for age.
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spelling pubmed-25703352008-10-29 Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults Dent, Arlene E. Bergmann-Leitner, Elke S. Wilson, Danny W. Tisch, Daniel J. Kimmel, Rhonda Vulule, John Sumba, Peter Odada Beeson, James G. Angov, Evelina Moormann, Ann M. Kazura, James W. PLoS One Research Article BACKGROUND: Antibodies that impair Plasmodium falciparum merozoite invasion and intraerythrocytic development are one of several mechanisms that mediate naturally acquired immunity to malaria. Attempts to correlate anti-malaria antibodies with risk of infection and morbidity have yielded inconsistent results. Growth inhibition assays (GIA) offer a convenient method to quantify functional antibody activity against blood stage malaria. METHODS: A treatment-time-to-infection study was conducted over 12-weeks in a malaria holoendemic area of Kenya. Plasma collected from healthy individuals (98 children and 99 adults) before artemether-lumefantrine treatment was tested by GIA in three separate laboratories. RESULTS: Median GIA levels varied with P. falciparum line (D10, 8.8%; 3D7, 34.9%; FVO, 51.4% inhibition). The magnitude of growth inhibition decreased with age in all P. falciparum lines tested with the highest median levels among children <4 years compared to adults (e.g. 3D7, 45.4% vs. 30.0% respectively, p = 0.0003). Time-to-infection measured by weekly blood smears was significantly associated with level of GIA controlling for age. Upper quartile inhibition activity was associated with less risk of infection compared to individuals with lower levels (e.g. 3D7, hazard ratio = 1.535, 95% CI = 1.012–2.329; p = 0.0438). Various GIA methodologies had little effect on measured parasite growth inhibition. CONCLUSION: Plasma antibody-mediated growth inhibition of blood stage P. falciparum decreases with age in residents of a malaria holoendemic area. Growth inhibition assay may be a useful surrogate of protection against infection when outcome is controlled for age. Public Library of Science 2008-10-29 /pmc/articles/PMC2570335/ /pubmed/18958285 http://dx.doi.org/10.1371/journal.pone.0003557 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Dent, Arlene E.
Bergmann-Leitner, Elke S.
Wilson, Danny W.
Tisch, Daniel J.
Kimmel, Rhonda
Vulule, John
Sumba, Peter Odada
Beeson, James G.
Angov, Evelina
Moormann, Ann M.
Kazura, James W.
Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults
title Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults
title_full Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults
title_fullStr Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults
title_full_unstemmed Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults
title_short Antibody-Mediated Growth Inhibition of Plasmodium falciparum: Relationship to Age and Protection from Parasitemia in Kenyan Children and Adults
title_sort antibody-mediated growth inhibition of plasmodium falciparum: relationship to age and protection from parasitemia in kenyan children and adults
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570335/
https://www.ncbi.nlm.nih.gov/pubmed/18958285
http://dx.doi.org/10.1371/journal.pone.0003557
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