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Enumeration of Mycobacterium leprae Using Real-Time PCR
Mycobacterium leprae is not cultivable in axenic media, and direct microscopic enumeration of the bacilli is complex, labor intensive, and suffers from limited sensitivity and specificity. We have developed a real-time PCR assay for quantifying M. leprae DNA in biological samples. Primers were ident...
Autores principales: | Truman, Richard W., Andrews, P. Kyle, Robbins, Naoko Y., Adams, Linda B., Krahenbuhl, James L., Gillis, Thomas P. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570796/ https://www.ncbi.nlm.nih.gov/pubmed/18982056 http://dx.doi.org/10.1371/journal.pntd.0000328 |
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