Cargando…

A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System

Unlike other monoamine neurotransmitters, the mechanism by which the brain's histamine content is regulated remains unclear. In mammals, vesicular monoamine transporters (VMATs) are expressed exclusively in neurons and mediate the storage of histamine and other monoamines. We have studied the v...

Descripción completa

Detalles Bibliográficos
Autores principales: Romero-Calderón, Rafael, Uhlenbrock, Guido, Borycz, Jolanta, Simon, Anne F., Grygoruk, Anna, Yee, Susan K., Shyer, Amy, Ackerson, Larry C., Maidment, Nigel T., Meinertzhagen, Ian A., Hovemann, Bernhard T., Krantz, David E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570955/
https://www.ncbi.nlm.nih.gov/pubmed/18989452
http://dx.doi.org/10.1371/journal.pgen.1000245
_version_ 1782160198424592384
author Romero-Calderón, Rafael
Uhlenbrock, Guido
Borycz, Jolanta
Simon, Anne F.
Grygoruk, Anna
Yee, Susan K.
Shyer, Amy
Ackerson, Larry C.
Maidment, Nigel T.
Meinertzhagen, Ian A.
Hovemann, Bernhard T.
Krantz, David E.
author_facet Romero-Calderón, Rafael
Uhlenbrock, Guido
Borycz, Jolanta
Simon, Anne F.
Grygoruk, Anna
Yee, Susan K.
Shyer, Amy
Ackerson, Larry C.
Maidment, Nigel T.
Meinertzhagen, Ian A.
Hovemann, Bernhard T.
Krantz, David E.
author_sort Romero-Calderón, Rafael
collection PubMed
description Unlike other monoamine neurotransmitters, the mechanism by which the brain's histamine content is regulated remains unclear. In mammals, vesicular monoamine transporters (VMATs) are expressed exclusively in neurons and mediate the storage of histamine and other monoamines. We have studied the visual system of Drosophila melanogaster in which histamine is the primary neurotransmitter released from photoreceptor cells. We report here that a novel mRNA splice variant of Drosophila VMAT (DVMAT-B) is expressed not in neurons but rather in a small subset of glia in the lamina of the fly's optic lobe. Histamine contents are reduced by mutation of dVMAT, but can be partially restored by specifically expressing DVMAT-B in glia. Our results suggest a novel role for a monoamine transporter in glia that may be relevant to histamine homeostasis in other systems.
format Text
id pubmed-2570955
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-25709552008-11-07 A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System Romero-Calderón, Rafael Uhlenbrock, Guido Borycz, Jolanta Simon, Anne F. Grygoruk, Anna Yee, Susan K. Shyer, Amy Ackerson, Larry C. Maidment, Nigel T. Meinertzhagen, Ian A. Hovemann, Bernhard T. Krantz, David E. PLoS Genet Research Article Unlike other monoamine neurotransmitters, the mechanism by which the brain's histamine content is regulated remains unclear. In mammals, vesicular monoamine transporters (VMATs) are expressed exclusively in neurons and mediate the storage of histamine and other monoamines. We have studied the visual system of Drosophila melanogaster in which histamine is the primary neurotransmitter released from photoreceptor cells. We report here that a novel mRNA splice variant of Drosophila VMAT (DVMAT-B) is expressed not in neurons but rather in a small subset of glia in the lamina of the fly's optic lobe. Histamine contents are reduced by mutation of dVMAT, but can be partially restored by specifically expressing DVMAT-B in glia. Our results suggest a novel role for a monoamine transporter in glia that may be relevant to histamine homeostasis in other systems. Public Library of Science 2008-11-07 /pmc/articles/PMC2570955/ /pubmed/18989452 http://dx.doi.org/10.1371/journal.pgen.1000245 Text en Romero-Calderón et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Romero-Calderón, Rafael
Uhlenbrock, Guido
Borycz, Jolanta
Simon, Anne F.
Grygoruk, Anna
Yee, Susan K.
Shyer, Amy
Ackerson, Larry C.
Maidment, Nigel T.
Meinertzhagen, Ian A.
Hovemann, Bernhard T.
Krantz, David E.
A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System
title A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System
title_full A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System
title_fullStr A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System
title_full_unstemmed A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System
title_short A Glial Variant of the Vesicular Monoamine Transporter Is Required To Store Histamine in the Drosophila Visual System
title_sort glial variant of the vesicular monoamine transporter is required to store histamine in the drosophila visual system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570955/
https://www.ncbi.nlm.nih.gov/pubmed/18989452
http://dx.doi.org/10.1371/journal.pgen.1000245
work_keys_str_mv AT romerocalderonrafael aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT uhlenbrockguido aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT boryczjolanta aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT simonannef aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT grygorukanna aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT yeesusank aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT shyeramy aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT ackersonlarryc aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT maidmentnigelt aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT meinertzhageniana aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT hovemannbernhardt aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT krantzdavide aglialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT romerocalderonrafael glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT uhlenbrockguido glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT boryczjolanta glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT simonannef glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT grygorukanna glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT yeesusank glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT shyeramy glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT ackersonlarryc glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT maidmentnigelt glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT meinertzhageniana glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT hovemannbernhardt glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem
AT krantzdavide glialvariantofthevesicularmonoaminetransporterisrequiredtostorehistamineinthedrosophilavisualsystem