Cargando…
A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
MicroRNA (miRNA) dysregulation frequently occurs in cancer. Analysis of whole blood miRNA in tumor models has not been widely reported, but could potentially lead to novel assays for early detection and monitoring of cancer. To determine whether miRNAs associated with malignancy could be detected in...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572631/ https://www.ncbi.nlm.nih.gov/pubmed/18826639 http://dx.doi.org/10.1186/1476-4598-7-74 |
_version_ | 1782160264298233856 |
---|---|
author | Fan, Alice C Goldrick, Marianna M Ho, Jennifer Liang, Yu Bachireddy, Pavan Felsher, Dean W |
author_facet | Fan, Alice C Goldrick, Marianna M Ho, Jennifer Liang, Yu Bachireddy, Pavan Felsher, Dean W |
author_sort | Fan, Alice C |
collection | PubMed |
description | MicroRNA (miRNA) dysregulation frequently occurs in cancer. Analysis of whole blood miRNA in tumor models has not been widely reported, but could potentially lead to novel assays for early detection and monitoring of cancer. To determine whether miRNAs associated with malignancy could be detected in the peripheral blood, we used real-time reverse transcriptase-PCR to determine miRNA profiles in whole blood obtained from transgenic mice with c-MYC-induced lymphoma, hepatocellular carcinoma and osteosarcoma. The PCR-based assays used in our studies require only 10 nanograms of total RNA, allowing serial mini-profiles (20 – 30 miRNAs) to be carried out on individual animals over time. Blood miRNAs were measured from mice at different stages of MYC-induced lymphomagenesis and regression. Unsupervised hierarchical clustering of the data identified specific miRNA expression profiles that correlated with tumor type and stage. The miRNAs found to be altered in the blood of mice with tumors frequently reverted to normal levels upon tumor regression. Our results suggest that specific changes in blood miRNA can be detected during tumorigenesis and tumor regression. |
format | Text |
id | pubmed-2572631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-25726312008-10-25 A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice Fan, Alice C Goldrick, Marianna M Ho, Jennifer Liang, Yu Bachireddy, Pavan Felsher, Dean W Mol Cancer Short Communication MicroRNA (miRNA) dysregulation frequently occurs in cancer. Analysis of whole blood miRNA in tumor models has not been widely reported, but could potentially lead to novel assays for early detection and monitoring of cancer. To determine whether miRNAs associated with malignancy could be detected in the peripheral blood, we used real-time reverse transcriptase-PCR to determine miRNA profiles in whole blood obtained from transgenic mice with c-MYC-induced lymphoma, hepatocellular carcinoma and osteosarcoma. The PCR-based assays used in our studies require only 10 nanograms of total RNA, allowing serial mini-profiles (20 – 30 miRNAs) to be carried out on individual animals over time. Blood miRNAs were measured from mice at different stages of MYC-induced lymphomagenesis and regression. Unsupervised hierarchical clustering of the data identified specific miRNA expression profiles that correlated with tumor type and stage. The miRNAs found to be altered in the blood of mice with tumors frequently reverted to normal levels upon tumor regression. Our results suggest that specific changes in blood miRNA can be detected during tumorigenesis and tumor regression. BioMed Central 2008-09-30 /pmc/articles/PMC2572631/ /pubmed/18826639 http://dx.doi.org/10.1186/1476-4598-7-74 Text en Copyright © 2008 Fan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Fan, Alice C Goldrick, Marianna M Ho, Jennifer Liang, Yu Bachireddy, Pavan Felsher, Dean W A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice |
title | A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice |
title_full | A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice |
title_fullStr | A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice |
title_full_unstemmed | A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice |
title_short | A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice |
title_sort | quantitative pcr method to detect blood micrornas associated with tumorigenesis in transgenic mice |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572631/ https://www.ncbi.nlm.nih.gov/pubmed/18826639 http://dx.doi.org/10.1186/1476-4598-7-74 |
work_keys_str_mv | AT fanalicec aquantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT goldrickmariannam aquantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT hojennifer aquantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT liangyu aquantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT bachireddypavan aquantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT felsherdeanw aquantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT fanalicec quantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT goldrickmariannam quantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT hojennifer quantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT liangyu quantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT bachireddypavan quantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice AT felsherdeanw quantitativepcrmethodtodetectbloodmicrornasassociatedwithtumorigenesisintransgenicmice |