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A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice

MicroRNA (miRNA) dysregulation frequently occurs in cancer. Analysis of whole blood miRNA in tumor models has not been widely reported, but could potentially lead to novel assays for early detection and monitoring of cancer. To determine whether miRNAs associated with malignancy could be detected in...

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Autores principales: Fan, Alice C, Goldrick, Marianna M, Ho, Jennifer, Liang, Yu, Bachireddy, Pavan, Felsher, Dean W
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572631/
https://www.ncbi.nlm.nih.gov/pubmed/18826639
http://dx.doi.org/10.1186/1476-4598-7-74
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author Fan, Alice C
Goldrick, Marianna M
Ho, Jennifer
Liang, Yu
Bachireddy, Pavan
Felsher, Dean W
author_facet Fan, Alice C
Goldrick, Marianna M
Ho, Jennifer
Liang, Yu
Bachireddy, Pavan
Felsher, Dean W
author_sort Fan, Alice C
collection PubMed
description MicroRNA (miRNA) dysregulation frequently occurs in cancer. Analysis of whole blood miRNA in tumor models has not been widely reported, but could potentially lead to novel assays for early detection and monitoring of cancer. To determine whether miRNAs associated with malignancy could be detected in the peripheral blood, we used real-time reverse transcriptase-PCR to determine miRNA profiles in whole blood obtained from transgenic mice with c-MYC-induced lymphoma, hepatocellular carcinoma and osteosarcoma. The PCR-based assays used in our studies require only 10 nanograms of total RNA, allowing serial mini-profiles (20 – 30 miRNAs) to be carried out on individual animals over time. Blood miRNAs were measured from mice at different stages of MYC-induced lymphomagenesis and regression. Unsupervised hierarchical clustering of the data identified specific miRNA expression profiles that correlated with tumor type and stage. The miRNAs found to be altered in the blood of mice with tumors frequently reverted to normal levels upon tumor regression. Our results suggest that specific changes in blood miRNA can be detected during tumorigenesis and tumor regression.
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spelling pubmed-25726312008-10-25 A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice Fan, Alice C Goldrick, Marianna M Ho, Jennifer Liang, Yu Bachireddy, Pavan Felsher, Dean W Mol Cancer Short Communication MicroRNA (miRNA) dysregulation frequently occurs in cancer. Analysis of whole blood miRNA in tumor models has not been widely reported, but could potentially lead to novel assays for early detection and monitoring of cancer. To determine whether miRNAs associated with malignancy could be detected in the peripheral blood, we used real-time reverse transcriptase-PCR to determine miRNA profiles in whole blood obtained from transgenic mice with c-MYC-induced lymphoma, hepatocellular carcinoma and osteosarcoma. The PCR-based assays used in our studies require only 10 nanograms of total RNA, allowing serial mini-profiles (20 – 30 miRNAs) to be carried out on individual animals over time. Blood miRNAs were measured from mice at different stages of MYC-induced lymphomagenesis and regression. Unsupervised hierarchical clustering of the data identified specific miRNA expression profiles that correlated with tumor type and stage. The miRNAs found to be altered in the blood of mice with tumors frequently reverted to normal levels upon tumor regression. Our results suggest that specific changes in blood miRNA can be detected during tumorigenesis and tumor regression. BioMed Central 2008-09-30 /pmc/articles/PMC2572631/ /pubmed/18826639 http://dx.doi.org/10.1186/1476-4598-7-74 Text en Copyright © 2008 Fan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Fan, Alice C
Goldrick, Marianna M
Ho, Jennifer
Liang, Yu
Bachireddy, Pavan
Felsher, Dean W
A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
title A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
title_full A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
title_fullStr A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
title_full_unstemmed A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
title_short A quantitative PCR method to detect blood microRNAs associated with tumorigenesis in transgenic mice
title_sort quantitative pcr method to detect blood micrornas associated with tumorigenesis in transgenic mice
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572631/
https://www.ncbi.nlm.nih.gov/pubmed/18826639
http://dx.doi.org/10.1186/1476-4598-7-74
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