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Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation
The cytokinetic furrow arises from spatial and temporal regulation of cortical contractility. To test the role microtubules play in furrow specification, we studied myosin II activation in echinoderm zygotes by assessing serine19-phosphorylated regulatory light chain (pRLC) localization after precis...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575787/ https://www.ncbi.nlm.nih.gov/pubmed/18955555 http://dx.doi.org/10.1083/jcb.200807128 |
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author | Foe, Victoria E. von Dassow, George |
author_facet | Foe, Victoria E. von Dassow, George |
author_sort | Foe, Victoria E. |
collection | PubMed |
description | The cytokinetic furrow arises from spatial and temporal regulation of cortical contractility. To test the role microtubules play in furrow specification, we studied myosin II activation in echinoderm zygotes by assessing serine19-phosphorylated regulatory light chain (pRLC) localization after precisely timed drug treatments. Cortical pRLC was globally depressed before cytokinesis, then elevated only at the equator. We implicated cell cycle biochemistry (not microtubules) in pRLC depression, and differential microtubule stability in localizing the subsequent myosin activation. With no microtubules, pRLC accumulation occurred globally instead of equatorially, and loss of just dynamic microtubules increased equatorial pRLC recruitment. Nocodazole treatment revealed a population of stable astral microtubules that formed during anaphase; among these, those aimed toward the equator grew longer, and their tips coincided with cortical pRLC accumulation. Shrinking the mitotic apparatus with colchicine revealed pRLC suppression near dynamic microtubule arrays. We conclude that opposite effects of stable versus dynamic microtubules focuses myosin activation to the cell equator during cytokinesis. |
format | Text |
id | pubmed-2575787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25757872009-05-03 Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation Foe, Victoria E. von Dassow, George J Cell Biol Research Articles The cytokinetic furrow arises from spatial and temporal regulation of cortical contractility. To test the role microtubules play in furrow specification, we studied myosin II activation in echinoderm zygotes by assessing serine19-phosphorylated regulatory light chain (pRLC) localization after precisely timed drug treatments. Cortical pRLC was globally depressed before cytokinesis, then elevated only at the equator. We implicated cell cycle biochemistry (not microtubules) in pRLC depression, and differential microtubule stability in localizing the subsequent myosin activation. With no microtubules, pRLC accumulation occurred globally instead of equatorially, and loss of just dynamic microtubules increased equatorial pRLC recruitment. Nocodazole treatment revealed a population of stable astral microtubules that formed during anaphase; among these, those aimed toward the equator grew longer, and their tips coincided with cortical pRLC accumulation. Shrinking the mitotic apparatus with colchicine revealed pRLC suppression near dynamic microtubule arrays. We conclude that opposite effects of stable versus dynamic microtubules focuses myosin activation to the cell equator during cytokinesis. The Rockefeller University Press 2008-11-03 /pmc/articles/PMC2575787/ /pubmed/18955555 http://dx.doi.org/10.1083/jcb.200807128 Text en © 2008 Foe and Dassow This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Foe, Victoria E. von Dassow, George Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation |
title | Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation |
title_full | Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation |
title_fullStr | Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation |
title_full_unstemmed | Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation |
title_short | Stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation |
title_sort | stable and dynamic microtubules coordinately shape the myosin activation zone during cytokinetic furrow formation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575787/ https://www.ncbi.nlm.nih.gov/pubmed/18955555 http://dx.doi.org/10.1083/jcb.200807128 |
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