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An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning

From experiments by Foe and von Dassow (Foe, V.E., and G. von Dassow. 2008. J. Cell Biol. 183:457–470) and others, we infer a molecular mechanism for positioning the cleavage furrow during cytokinesis. Computer simulations reveal how this mechanism depends on quantitative motor-behavior details and...

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Detalles Bibliográficos
Autores principales: Odell, Garrett M., Foe, Victoria E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575788/
https://www.ncbi.nlm.nih.gov/pubmed/18955556
http://dx.doi.org/10.1083/jcb.200807129
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author Odell, Garrett M.
Foe, Victoria E.
author_facet Odell, Garrett M.
Foe, Victoria E.
author_sort Odell, Garrett M.
collection PubMed
description From experiments by Foe and von Dassow (Foe, V.E., and G. von Dassow. 2008. J. Cell Biol. 183:457–470) and others, we infer a molecular mechanism for positioning the cleavage furrow during cytokinesis. Computer simulations reveal how this mechanism depends on quantitative motor-behavior details and explore how robustly this mechanism succeeds across a range of cell sizes. The mechanism involves the MKLP1 (kinesin-6) component of centralspindlin binding to and walking along microtubules to stimulate cortical contractility where the centralspindlin complex concentrates. The majority of astral microtubules are dynamically unstable. They bind most MKLP1 and suppress cortical Rho/myosin II activation because the tips of unstable microtubules usually depolymerize before MKLP1s reach the cortex. A subset of astral microtubules stabilizes during anaphase, becoming effective rails along which MKLP1 can actually reach the cortex. Because stabilized microtubules aim statistically at the equatorial spindle midplane, that is where centralspindlin accumulates to stimulate furrow formation.
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spelling pubmed-25757882009-05-03 An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning Odell, Garrett M. Foe, Victoria E. J Cell Biol Research Articles From experiments by Foe and von Dassow (Foe, V.E., and G. von Dassow. 2008. J. Cell Biol. 183:457–470) and others, we infer a molecular mechanism for positioning the cleavage furrow during cytokinesis. Computer simulations reveal how this mechanism depends on quantitative motor-behavior details and explore how robustly this mechanism succeeds across a range of cell sizes. The mechanism involves the MKLP1 (kinesin-6) component of centralspindlin binding to and walking along microtubules to stimulate cortical contractility where the centralspindlin complex concentrates. The majority of astral microtubules are dynamically unstable. They bind most MKLP1 and suppress cortical Rho/myosin II activation because the tips of unstable microtubules usually depolymerize before MKLP1s reach the cortex. A subset of astral microtubules stabilizes during anaphase, becoming effective rails along which MKLP1 can actually reach the cortex. Because stabilized microtubules aim statistically at the equatorial spindle midplane, that is where centralspindlin accumulates to stimulate furrow formation. The Rockefeller University Press 2008-11-03 /pmc/articles/PMC2575788/ /pubmed/18955556 http://dx.doi.org/10.1083/jcb.200807129 Text en © 2008 Odell and Foe This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Odell, Garrett M.
Foe, Victoria E.
An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning
title An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning
title_full An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning
title_fullStr An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning
title_full_unstemmed An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning
title_short An agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning
title_sort agent-based model contrasts opposite effects of dynamic and stable microtubules on cleavage furrow positioning
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575788/
https://www.ncbi.nlm.nih.gov/pubmed/18955556
http://dx.doi.org/10.1083/jcb.200807129
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