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The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution

Scrub typhus (‘Tsutsugamushi’ disease in Japanese) is a mite-borne infectious disease. The causative agent is Orientia tsutsugamushi, an obligate intracellular bacterium belonging to the family Rickettsiaceae of the subdivision alpha-Proteobacteria. In this study, we determined the complete genome s...

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Autores principales: Nakayama, Keisuke, Yamashita, Atsushi, Kurokawa, Ken, Morimoto, Takuya, Ogawa, Michihiro, Fukuhara, Masahiro, Urakami, Hiroshi, Ohnishi, Makoto, Uchiyama, Ikuo, Ogura, Yoshitoshi, Ooka, Tadasuke, Oshima, Kenshiro, Tamura, Akira, Hattori, Masahira, Hayashi, Tetsuya
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575882/
https://www.ncbi.nlm.nih.gov/pubmed/18508905
http://dx.doi.org/10.1093/dnares/dsn011
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author Nakayama, Keisuke
Yamashita, Atsushi
Kurokawa, Ken
Morimoto, Takuya
Ogawa, Michihiro
Fukuhara, Masahiro
Urakami, Hiroshi
Ohnishi, Makoto
Uchiyama, Ikuo
Ogura, Yoshitoshi
Ooka, Tadasuke
Oshima, Kenshiro
Tamura, Akira
Hattori, Masahira
Hayashi, Tetsuya
author_facet Nakayama, Keisuke
Yamashita, Atsushi
Kurokawa, Ken
Morimoto, Takuya
Ogawa, Michihiro
Fukuhara, Masahiro
Urakami, Hiroshi
Ohnishi, Makoto
Uchiyama, Ikuo
Ogura, Yoshitoshi
Ooka, Tadasuke
Oshima, Kenshiro
Tamura, Akira
Hattori, Masahira
Hayashi, Tetsuya
author_sort Nakayama, Keisuke
collection PubMed
description Scrub typhus (‘Tsutsugamushi’ disease in Japanese) is a mite-borne infectious disease. The causative agent is Orientia tsutsugamushi, an obligate intracellular bacterium belonging to the family Rickettsiaceae of the subdivision alpha-Proteobacteria. In this study, we determined the complete genome sequence of O. tsutsugamushi strain Ikeda, which comprises a single chromosome of 2 008 987 bp and contains 1967 protein coding sequences (CDSs). The chromosome is much larger than those of other members of Rickettsiaceae, and 46.7% of the sequence was occupied by repetitive sequences derived from an integrative and conjugative element, 10 types of transposable elements, and seven types of short repeats of unknown origins. The massive amplification and degradation of these elements have generated a huge number of repeated genes (1196 CDSs, categorized into 85 families), many of which are pseudogenes (766 CDSs), and also induced intensive genome shuffling. By comparing the gene content with those of other family members of Rickettsiacea, we identified the core gene set of the family Rickettsiaceae and found that, while much more extensive gene loss has taken place among the housekeeping genes of Orientia than those of Rickettsia, O. tsutsugamushi has acquired a large number of foreign genes. The O. tsutsugamushi genome sequence is thus a prominent example of the high plasticity of bacterial genomes, and provides the genetic basis for a better understanding of the biology of O. tsutsugamushi and the pathogenesis of ‘Tsutsugamushi’ disease.
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spelling pubmed-25758822009-04-13 The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution Nakayama, Keisuke Yamashita, Atsushi Kurokawa, Ken Morimoto, Takuya Ogawa, Michihiro Fukuhara, Masahiro Urakami, Hiroshi Ohnishi, Makoto Uchiyama, Ikuo Ogura, Yoshitoshi Ooka, Tadasuke Oshima, Kenshiro Tamura, Akira Hattori, Masahira Hayashi, Tetsuya DNA Res Full Papers Scrub typhus (‘Tsutsugamushi’ disease in Japanese) is a mite-borne infectious disease. The causative agent is Orientia tsutsugamushi, an obligate intracellular bacterium belonging to the family Rickettsiaceae of the subdivision alpha-Proteobacteria. In this study, we determined the complete genome sequence of O. tsutsugamushi strain Ikeda, which comprises a single chromosome of 2 008 987 bp and contains 1967 protein coding sequences (CDSs). The chromosome is much larger than those of other members of Rickettsiaceae, and 46.7% of the sequence was occupied by repetitive sequences derived from an integrative and conjugative element, 10 types of transposable elements, and seven types of short repeats of unknown origins. The massive amplification and degradation of these elements have generated a huge number of repeated genes (1196 CDSs, categorized into 85 families), many of which are pseudogenes (766 CDSs), and also induced intensive genome shuffling. By comparing the gene content with those of other family members of Rickettsiacea, we identified the core gene set of the family Rickettsiaceae and found that, while much more extensive gene loss has taken place among the housekeeping genes of Orientia than those of Rickettsia, O. tsutsugamushi has acquired a large number of foreign genes. The O. tsutsugamushi genome sequence is thus a prominent example of the high plasticity of bacterial genomes, and provides the genetic basis for a better understanding of the biology of O. tsutsugamushi and the pathogenesis of ‘Tsutsugamushi’ disease. Oxford University Press 2008-08 2008-05-28 /pmc/articles/PMC2575882/ /pubmed/18508905 http://dx.doi.org/10.1093/dnares/dsn011 Text en © The Author 2008. Kazusa DNA Research Institute
spellingShingle Full Papers
Nakayama, Keisuke
Yamashita, Atsushi
Kurokawa, Ken
Morimoto, Takuya
Ogawa, Michihiro
Fukuhara, Masahiro
Urakami, Hiroshi
Ohnishi, Makoto
Uchiyama, Ikuo
Ogura, Yoshitoshi
Ooka, Tadasuke
Oshima, Kenshiro
Tamura, Akira
Hattori, Masahira
Hayashi, Tetsuya
The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
title The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
title_full The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
title_fullStr The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
title_full_unstemmed The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
title_short The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
title_sort whole-genome sequencing of the obligate intracellular bacterium orientia tsutsugamushi revealed massive gene amplification during reductive genome evolution
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575882/
https://www.ncbi.nlm.nih.gov/pubmed/18508905
http://dx.doi.org/10.1093/dnares/dsn011
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