A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers

A collection of 94 F(6) individuals derived from crosses between Lotus japonicus, Gifu B-129 (G) and Miyakojima MG-20 (M) were used for mapping. By using the HEGS running system, 427 EcoRI/MseI primer pairs were selected to generate a total of 2053 markers, consisting of 739 G-associated dominant ma...

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Detalles Bibliográficos
Autores principales: Wang, Xinwang, Sato, Shusei, Tabata, Satoshi, Kawasaki, Shinji
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575890/
https://www.ncbi.nlm.nih.gov/pubmed/18794159
http://dx.doi.org/10.1093/dnares/dsn022
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author Wang, Xinwang
Sato, Shusei
Tabata, Satoshi
Kawasaki, Shinji
author_facet Wang, Xinwang
Sato, Shusei
Tabata, Satoshi
Kawasaki, Shinji
author_sort Wang, Xinwang
collection PubMed
description A collection of 94 F(6) individuals derived from crosses between Lotus japonicus, Gifu B-129 (G) and Miyakojima MG-20 (M) were used for mapping. By using the HEGS running system, 427 EcoRI/MseI primer pairs were selected to generate a total of 2053 markers, consisting of 739 G-associated dominant markers, 674 M-associated dominant markers, 640 co-dominant markers, 95 SSR markers and 2 dCAPS markers. Excluding heavily distorted markers, 1588 were mapped to six chromosomes of the L. japonicus genome based on the 97 reference markers. This linkage map consisted of 1023 unique markers (excluding duplicated markers) and covered a total of 508.5 cM of the genome with an average chromosome length of 84.7 cM and interval distance of 0.50 cM. Fifteen quantitative traits loci for eight morphological traits were also mapped. This linkage map will provide a useful framework for physical map construction in L. japonicus in the near future.
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spelling pubmed-25758902009-04-13 A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers Wang, Xinwang Sato, Shusei Tabata, Satoshi Kawasaki, Shinji DNA Res Full Papers A collection of 94 F(6) individuals derived from crosses between Lotus japonicus, Gifu B-129 (G) and Miyakojima MG-20 (M) were used for mapping. By using the HEGS running system, 427 EcoRI/MseI primer pairs were selected to generate a total of 2053 markers, consisting of 739 G-associated dominant markers, 674 M-associated dominant markers, 640 co-dominant markers, 95 SSR markers and 2 dCAPS markers. Excluding heavily distorted markers, 1588 were mapped to six chromosomes of the L. japonicus genome based on the 97 reference markers. This linkage map consisted of 1023 unique markers (excluding duplicated markers) and covered a total of 508.5 cM of the genome with an average chromosome length of 84.7 cM and interval distance of 0.50 cM. Fifteen quantitative traits loci for eight morphological traits were also mapped. This linkage map will provide a useful framework for physical map construction in L. japonicus in the near future. Oxford University Press 2008-10 2008-09-14 /pmc/articles/PMC2575890/ /pubmed/18794159 http://dx.doi.org/10.1093/dnares/dsn022 Text en © The Author 2008. Kazusa DNA Research Institute
spellingShingle Full Papers
Wang, Xinwang
Sato, Shusei
Tabata, Satoshi
Kawasaki, Shinji
A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers
title A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers
title_full A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers
title_fullStr A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers
title_full_unstemmed A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers
title_short A High-density Linkage Map of Lotus japonicus Based on AFLP and SSR Markers
title_sort high-density linkage map of lotus japonicus based on aflp and ssr markers
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2575890/
https://www.ncbi.nlm.nih.gov/pubmed/18794159
http://dx.doi.org/10.1093/dnares/dsn022
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