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Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate

BACKGROUND: The restoration of adults from fragments of blood vessels in botryllid ascidians (termed whole body regeneration [WBR]) represents an inimitable event in the chordates, which is poorly understood on the mechanistic level. RESULTS: To elucidate mechanisms underlying this phenomenon, a sub...

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Autores principales: Rinkevich, Yuval, Rinkevich, Baruch, Reshef, Ram
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2576188/
https://www.ncbi.nlm.nih.gov/pubmed/18847507
http://dx.doi.org/10.1186/1471-213X-8-100
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author Rinkevich, Yuval
Rinkevich, Baruch
Reshef, Ram
author_facet Rinkevich, Yuval
Rinkevich, Baruch
Reshef, Ram
author_sort Rinkevich, Yuval
collection PubMed
description BACKGROUND: The restoration of adults from fragments of blood vessels in botryllid ascidians (termed whole body regeneration [WBR]) represents an inimitable event in the chordates, which is poorly understood on the mechanistic level. RESULTS: To elucidate mechanisms underlying this phenomenon, a subtracted EST library for early WBR stages was previously assembled, revealing 76 putative genes belonging to major signaling pathways, including Notch/Delta, JAK/STAT, protein kinases, nuclear receptors, Ras oncogene family members, G-Protein coupled receptor (GPCR) and transforming growth factor beta (TGF-β) signaling. RT-PCR on selected transcripts documented specific up-regulation in only regenerating fragments, pointing to a broad activation of these signaling pathways at onset of WBR. The followed-up expression pattern of seven representative transcripts from JAK/STAT signaling (Bl-STAT), the Ras oncogene family (Bl-Rap1A, Bl-Rab-33), the protein kinase family (Bl-Mnk), Bl-Cnot, Bl-Slit and Bl-Bax inhibitor, revealed systemic and site specific activations during WBR in a sub-population of circulatory cells. CONCLUSION: WBR in the non-vertebrate chordate Botrylloides leachi is a multifaceted phenomenon, presided by a complex array of cell signaling and transcription factors. Above results, provide a first insight into the whole genome molecular machinery of this unique regeneration process, and reveal the broad participation of cell signaling and transcription factors in the process. While regeneration involves the participation of specific cell populations, WBR signals are systemically expressed at the organism level.
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spelling pubmed-25761882008-10-31 Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate Rinkevich, Yuval Rinkevich, Baruch Reshef, Ram BMC Dev Biol Research Article BACKGROUND: The restoration of adults from fragments of blood vessels in botryllid ascidians (termed whole body regeneration [WBR]) represents an inimitable event in the chordates, which is poorly understood on the mechanistic level. RESULTS: To elucidate mechanisms underlying this phenomenon, a subtracted EST library for early WBR stages was previously assembled, revealing 76 putative genes belonging to major signaling pathways, including Notch/Delta, JAK/STAT, protein kinases, nuclear receptors, Ras oncogene family members, G-Protein coupled receptor (GPCR) and transforming growth factor beta (TGF-β) signaling. RT-PCR on selected transcripts documented specific up-regulation in only regenerating fragments, pointing to a broad activation of these signaling pathways at onset of WBR. The followed-up expression pattern of seven representative transcripts from JAK/STAT signaling (Bl-STAT), the Ras oncogene family (Bl-Rap1A, Bl-Rab-33), the protein kinase family (Bl-Mnk), Bl-Cnot, Bl-Slit and Bl-Bax inhibitor, revealed systemic and site specific activations during WBR in a sub-population of circulatory cells. CONCLUSION: WBR in the non-vertebrate chordate Botrylloides leachi is a multifaceted phenomenon, presided by a complex array of cell signaling and transcription factors. Above results, provide a first insight into the whole genome molecular machinery of this unique regeneration process, and reveal the broad participation of cell signaling and transcription factors in the process. While regeneration involves the participation of specific cell populations, WBR signals are systemically expressed at the organism level. BioMed Central 2008-10-12 /pmc/articles/PMC2576188/ /pubmed/18847507 http://dx.doi.org/10.1186/1471-213X-8-100 Text en Copyright © 2008 Rinkevich et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rinkevich, Yuval
Rinkevich, Baruch
Reshef, Ram
Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate
title Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate
title_full Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate
title_fullStr Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate
title_full_unstemmed Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate
title_short Cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate
title_sort cell signaling and transcription factor genes expressed during whole body regeneration in a colonial chordate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2576188/
https://www.ncbi.nlm.nih.gov/pubmed/18847507
http://dx.doi.org/10.1186/1471-213X-8-100
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