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Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2

In this research, we developed a novel chimeric HTNV-IL-2-G2 DNA vaccine plasmid by genetically linking IL-2 gene to the G2 segment DNA and tested whether it could be a candidate vaccine. Chimeric gene was first expressed in eukaryotic expression system pcDNA3.1 (+). The HTNV-IL-2-G2 expressed a 72...

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Autores principales: Hao, Huang, Xiu, Li, Zehua, Zhang, Min, Jia, Hongbo, Hu, Zhihong, Wu, Zhenhua, Zhu, Xiaohong, Wan, Hanju, Huang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577087/
https://www.ncbi.nlm.nih.gov/pubmed/18940009
http://dx.doi.org/10.1186/1479-0556-6-15
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author Hao, Huang
Xiu, Li
Zehua, Zhang
Min, Jia
Hongbo, Hu
Zhihong, Wu
Zhenhua, Zhu
Xiaohong, Wan
Hanju, Huang
author_facet Hao, Huang
Xiu, Li
Zehua, Zhang
Min, Jia
Hongbo, Hu
Zhihong, Wu
Zhenhua, Zhu
Xiaohong, Wan
Hanju, Huang
author_sort Hao, Huang
collection PubMed
description In this research, we developed a novel chimeric HTNV-IL-2-G2 DNA vaccine plasmid by genetically linking IL-2 gene to the G2 segment DNA and tested whether it could be a candidate vaccine. Chimeric gene was first expressed in eukaryotic expression system pcDNA3.1 (+). The HTNV-IL-2-G2 expressed a 72 kDa fusion protein in COS-7 cells. Meanwhile, the fusion protein kept the activity of its parental proteins. Furthermore, BALB/c mice were vaccinated by the chimeric gene. ELISA, cell microculture neutralization test in vitro were used to detect the humoral immune response in immunized BALB/c mice. Lymphocyte proliferation assay was used to detect the cellular immune response.- The results showed that the chimeric gene could simultaneously evoke specific antibody against G2 glycoprotein and IL-2. And the immunized mice of every group elicited neutralizing antibodies with different titers. Lymphocyte proliferation assay results showed that the stimulation indexes of splenocytes of chimeric gene to G2 and IL-2 were significantly higher than that of other groups. Our results suggest that IL-2-based HTNV G2 DNA can induce both humoral and cellular immune response specific for HTNV G2 and can be a candidate DNA vaccine for HTNV infection.
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spelling pubmed-25770872008-11-01 Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2 Hao, Huang Xiu, Li Zehua, Zhang Min, Jia Hongbo, Hu Zhihong, Wu Zhenhua, Zhu Xiaohong, Wan Hanju, Huang Genet Vaccines Ther Research In this research, we developed a novel chimeric HTNV-IL-2-G2 DNA vaccine plasmid by genetically linking IL-2 gene to the G2 segment DNA and tested whether it could be a candidate vaccine. Chimeric gene was first expressed in eukaryotic expression system pcDNA3.1 (+). The HTNV-IL-2-G2 expressed a 72 kDa fusion protein in COS-7 cells. Meanwhile, the fusion protein kept the activity of its parental proteins. Furthermore, BALB/c mice were vaccinated by the chimeric gene. ELISA, cell microculture neutralization test in vitro were used to detect the humoral immune response in immunized BALB/c mice. Lymphocyte proliferation assay was used to detect the cellular immune response.- The results showed that the chimeric gene could simultaneously evoke specific antibody against G2 glycoprotein and IL-2. And the immunized mice of every group elicited neutralizing antibodies with different titers. Lymphocyte proliferation assay results showed that the stimulation indexes of splenocytes of chimeric gene to G2 and IL-2 were significantly higher than that of other groups. Our results suggest that IL-2-based HTNV G2 DNA can induce both humoral and cellular immune response specific for HTNV G2 and can be a candidate DNA vaccine for HTNV infection. BioMed Central 2008-10-22 /pmc/articles/PMC2577087/ /pubmed/18940009 http://dx.doi.org/10.1186/1479-0556-6-15 Text en Copyright © 2008 Hao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hao, Huang
Xiu, Li
Zehua, Zhang
Min, Jia
Hongbo, Hu
Zhihong, Wu
Zhenhua, Zhu
Xiaohong, Wan
Hanju, Huang
Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2
title Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2
title_full Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2
title_fullStr Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2
title_full_unstemmed Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2
title_short Genetic immunization with Hantavirus vaccine combining expression of G2 glycoprotein and fused interleukin-2
title_sort genetic immunization with hantavirus vaccine combining expression of g2 glycoprotein and fused interleukin-2
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577087/
https://www.ncbi.nlm.nih.gov/pubmed/18940009
http://dx.doi.org/10.1186/1479-0556-6-15
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