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Extensive chromatin fragmentation improves enrichment of protein binding sites in chromatin immunoprecipitation experiments
Extensive sonication of formaldehyde-crosslinked chromatin can generate DNA fragments averaging 200 bp in length (range 75–300 bp). Fragmentation is largely random with respect to genomic region and nucleosome position. ChIP experiments employing such extensively fragmented samples show 2- to 4-fold...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577354/ https://www.ncbi.nlm.nih.gov/pubmed/18765474 http://dx.doi.org/10.1093/nar/gkn535 |