Cargando…
Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains
Polyploidy has had a considerable impact on the evolution of many eukaryotes, especially angiosperms. Indeed, most—if not all—angiosperms have experienced at least one round of polyploidy during the course of their evolution, and many important crop plants are current polyploids. The occurrence of 2...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2581889/ https://www.ncbi.nlm.nih.gov/pubmed/19043546 http://dx.doi.org/10.1371/journal.pgen.1000274 |
_version_ | 1782160647876771840 |
---|---|
author | d'Erfurth, Isabelle Jolivet, Sylvie Froger, Nicole Catrice, Olivier Novatchkova, Maria Simon, Mathieu Jenczewski, Eric Mercier, Raphaël |
author_facet | d'Erfurth, Isabelle Jolivet, Sylvie Froger, Nicole Catrice, Olivier Novatchkova, Maria Simon, Mathieu Jenczewski, Eric Mercier, Raphaël |
author_sort | d'Erfurth, Isabelle |
collection | PubMed |
description | Polyploidy has had a considerable impact on the evolution of many eukaryotes, especially angiosperms. Indeed, most—if not all—angiosperms have experienced at least one round of polyploidy during the course of their evolution, and many important crop plants are current polyploids. The occurrence of 2n gametes (diplogametes) in diploid populations is widely recognised as the major source of polyploid formation. However, limited information is available on the genetic control of diplogamete production. Here, we describe the isolation and characterisation of the first gene, AtPS1 (Arabidopsis thaliana Parallel Spindle 1), implicated in the formation of a high frequency of diplogametes in plants. Atps1 mutants produce diploid male spores, diploid pollen grains, and spontaneous triploid plants in the next generation. Female meiosis is not affected in the mutant. We demonstrated that abnormal spindle orientation at male meiosis II leads to diplogamete formation. Most of the parent's heterozygosity is therefore conserved in the Atps1 diploid gametes, which is a key issue for plant breeding. The AtPS1 protein is conserved throughout the plant kingdom and carries domains suggestive of a regulatory function. The isolation of a gene involved in diplogamete production opens the way for new strategies in plant breeding programmes and progress in evolutionary studies. |
format | Text |
id | pubmed-2581889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-25818892008-11-28 Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains d'Erfurth, Isabelle Jolivet, Sylvie Froger, Nicole Catrice, Olivier Novatchkova, Maria Simon, Mathieu Jenczewski, Eric Mercier, Raphaël PLoS Genet Research Article Polyploidy has had a considerable impact on the evolution of many eukaryotes, especially angiosperms. Indeed, most—if not all—angiosperms have experienced at least one round of polyploidy during the course of their evolution, and many important crop plants are current polyploids. The occurrence of 2n gametes (diplogametes) in diploid populations is widely recognised as the major source of polyploid formation. However, limited information is available on the genetic control of diplogamete production. Here, we describe the isolation and characterisation of the first gene, AtPS1 (Arabidopsis thaliana Parallel Spindle 1), implicated in the formation of a high frequency of diplogametes in plants. Atps1 mutants produce diploid male spores, diploid pollen grains, and spontaneous triploid plants in the next generation. Female meiosis is not affected in the mutant. We demonstrated that abnormal spindle orientation at male meiosis II leads to diplogamete formation. Most of the parent's heterozygosity is therefore conserved in the Atps1 diploid gametes, which is a key issue for plant breeding. The AtPS1 protein is conserved throughout the plant kingdom and carries domains suggestive of a regulatory function. The isolation of a gene involved in diplogamete production opens the way for new strategies in plant breeding programmes and progress in evolutionary studies. Public Library of Science 2008-11-28 /pmc/articles/PMC2581889/ /pubmed/19043546 http://dx.doi.org/10.1371/journal.pgen.1000274 Text en d'Erfurth et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article d'Erfurth, Isabelle Jolivet, Sylvie Froger, Nicole Catrice, Olivier Novatchkova, Maria Simon, Mathieu Jenczewski, Eric Mercier, Raphaël Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains |
title | Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains |
title_full | Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains |
title_fullStr | Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains |
title_full_unstemmed | Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains |
title_short | Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains |
title_sort | mutations in atps1 (arabidopsis thaliana parallel spindle 1) lead to the production of diploid pollen grains |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2581889/ https://www.ncbi.nlm.nih.gov/pubmed/19043546 http://dx.doi.org/10.1371/journal.pgen.1000274 |
work_keys_str_mv | AT derfurthisabelle mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains AT jolivetsylvie mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains AT frogernicole mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains AT catriceolivier mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains AT novatchkovamaria mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains AT simonmathieu mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains AT jenczewskieric mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains AT mercierraphael mutationsinatps1arabidopsisthalianaparallelspindle1leadtotheproductionofdiploidpollengrains |