Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)

Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sit...

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Autores principales: Lusic, Hrvoje, Gustilo, Estella M., Vendeix, Franck A.P., Kaiser, Rob, Delaney, Michael O., Graham, William D., Moye, Virginia A., Cantara, William A., Agris, Paul F., Deiters, Alexander
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582600/
https://www.ncbi.nlm.nih.gov/pubmed/18927116
http://dx.doi.org/10.1093/nar/gkn703
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author Lusic, Hrvoje
Gustilo, Estella M.
Vendeix, Franck A.P.
Kaiser, Rob
Delaney, Michael O.
Graham, William D.
Moye, Virginia A.
Cantara, William A.
Agris, Paul F.
Deiters, Alexander
author_facet Lusic, Hrvoje
Gustilo, Estella M.
Vendeix, Franck A.P.
Kaiser, Rob
Delaney, Michael O.
Graham, William D.
Moye, Virginia A.
Cantara, William A.
Agris, Paul F.
Deiters, Alexander
author_sort Lusic, Hrvoje
collection PubMed
description Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.
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spelling pubmed-25826002008-11-13 Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met) Lusic, Hrvoje Gustilo, Estella M. Vendeix, Franck A.P. Kaiser, Rob Delaney, Michael O. Graham, William D. Moye, Virginia A. Cantara, William A. Agris, Paul F. Deiters, Alexander Nucleic Acids Res RNA Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation. Oxford University Press 2008-11 2008-10-16 /pmc/articles/PMC2582600/ /pubmed/18927116 http://dx.doi.org/10.1093/nar/gkn703 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Lusic, Hrvoje
Gustilo, Estella M.
Vendeix, Franck A.P.
Kaiser, Rob
Delaney, Michael O.
Graham, William D.
Moye, Virginia A.
Cantara, William A.
Agris, Paul F.
Deiters, Alexander
Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)
title Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)
title_full Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)
title_fullStr Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)
title_full_unstemmed Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)
title_short Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNA(Met)
title_sort synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial trna(met)
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582600/
https://www.ncbi.nlm.nih.gov/pubmed/18927116
http://dx.doi.org/10.1093/nar/gkn703
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