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A kinesin-13 mutant catalytically depolymerizes microtubules in ADP

The kinesin-13 motor protein family members drive the removal of tubulin from microtubules (MTs) to promote MT turnover. A point mutation of the kinesin-13 family member mitotic centromere-associated kinesin/Kif2C (E491A) isolates the tubulin-removal conformation of the motor, and appears distinct f...

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Detalles Bibliográficos
Autores principales: Wagenbach, Michael, Domnitz, Sarah, Wordeman, Linda, Cooper, Jeremy
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582896/
https://www.ncbi.nlm.nih.gov/pubmed/19001124
http://dx.doi.org/10.1083/jcb.200805145
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author Wagenbach, Michael
Domnitz, Sarah
Wordeman, Linda
Cooper, Jeremy
author_facet Wagenbach, Michael
Domnitz, Sarah
Wordeman, Linda
Cooper, Jeremy
author_sort Wagenbach, Michael
collection PubMed
description The kinesin-13 motor protein family members drive the removal of tubulin from microtubules (MTs) to promote MT turnover. A point mutation of the kinesin-13 family member mitotic centromere-associated kinesin/Kif2C (E491A) isolates the tubulin-removal conformation of the motor, and appears distinct from all previously described kinesin-13 conformations derived from nucleotide analogues. The E491A mutant removes tubulin dimers from stabilized MTs stoichiometrically in adenosine triphosphate (ATP) but is unable to efficiently release from detached tubulin dimers to recycle catalytically. Only in adenosine diphosphate (ADP) can the mutant catalytically remove tubulin dimers from stabilized MTs because the affinity of the mutant for detached tubulin dimers in ADP is low relative to lattice-bound tubulin. Thus, the motor can regenerate for further cycles of disassembly. Using the mutant, we show that release of tubulin by kinesin-13 motors occurs at the transition state for ATP hydrolysis, which illustrates a significant divergence in their coupling to ATP turnover relative to motile kinesins.
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spelling pubmed-25828962009-05-18 A kinesin-13 mutant catalytically depolymerizes microtubules in ADP Wagenbach, Michael Domnitz, Sarah Wordeman, Linda Cooper, Jeremy J Cell Biol Research Articles The kinesin-13 motor protein family members drive the removal of tubulin from microtubules (MTs) to promote MT turnover. A point mutation of the kinesin-13 family member mitotic centromere-associated kinesin/Kif2C (E491A) isolates the tubulin-removal conformation of the motor, and appears distinct from all previously described kinesin-13 conformations derived from nucleotide analogues. The E491A mutant removes tubulin dimers from stabilized MTs stoichiometrically in adenosine triphosphate (ATP) but is unable to efficiently release from detached tubulin dimers to recycle catalytically. Only in adenosine diphosphate (ADP) can the mutant catalytically remove tubulin dimers from stabilized MTs because the affinity of the mutant for detached tubulin dimers in ADP is low relative to lattice-bound tubulin. Thus, the motor can regenerate for further cycles of disassembly. Using the mutant, we show that release of tubulin by kinesin-13 motors occurs at the transition state for ATP hydrolysis, which illustrates a significant divergence in their coupling to ATP turnover relative to motile kinesins. The Rockefeller University Press 2008-11-17 /pmc/articles/PMC2582896/ /pubmed/19001124 http://dx.doi.org/10.1083/jcb.200805145 Text en © 2008 Wagenbach et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Wagenbach, Michael
Domnitz, Sarah
Wordeman, Linda
Cooper, Jeremy
A kinesin-13 mutant catalytically depolymerizes microtubules in ADP
title A kinesin-13 mutant catalytically depolymerizes microtubules in ADP
title_full A kinesin-13 mutant catalytically depolymerizes microtubules in ADP
title_fullStr A kinesin-13 mutant catalytically depolymerizes microtubules in ADP
title_full_unstemmed A kinesin-13 mutant catalytically depolymerizes microtubules in ADP
title_short A kinesin-13 mutant catalytically depolymerizes microtubules in ADP
title_sort kinesin-13 mutant catalytically depolymerizes microtubules in adp
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582896/
https://www.ncbi.nlm.nih.gov/pubmed/19001124
http://dx.doi.org/10.1083/jcb.200805145
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