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SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation
De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Her...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582956/ https://www.ncbi.nlm.nih.gov/pubmed/19043555 http://dx.doi.org/10.1371/journal.pgen.1000280 |
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author | Johnson, Lianna M. Law, Julie A. Khattar, Anuj Henderson, Ian R. Jacobsen, Steven E. |
author_facet | Johnson, Lianna M. Law, Julie A. Khattar, Anuj Henderson, Ian R. Jacobsen, Steven E. |
author_sort | Johnson, Lianna M. |
collection | PubMed |
description | De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Here, we show that two SRA-domain proteins (SUVH9 and SUVH2) are also essential for DRM2-mediated de novo and maintenance DNA methylation in Arabidopsis thaliana. At some loci, SUVH9 and SUVH2 act redundantly, while at other loci only SUVH2 is required, and this locus specificity correlates with the differing DNA-binding affinity of the SRA domains within SUVH9 and SUVH2. Specifically, SUVH9 preferentially binds methylated asymmetric sites, while SUVH2 preferentially binds methylated CG sites. The suvh9 and suvh2 mutations do not eliminate siRNAs, suggesting a role for SUVH9 and SUVH2 late in the RNA-directed DNA methylation pathway. With these new results, it is clear that SRA-domain proteins are involved in each of the three pathways leading to DNA methylation in Arabidopsis. |
format | Text |
id | pubmed-2582956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-25829562008-11-28 SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation Johnson, Lianna M. Law, Julie A. Khattar, Anuj Henderson, Ian R. Jacobsen, Steven E. PLoS Genet Research Article De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Here, we show that two SRA-domain proteins (SUVH9 and SUVH2) are also essential for DRM2-mediated de novo and maintenance DNA methylation in Arabidopsis thaliana. At some loci, SUVH9 and SUVH2 act redundantly, while at other loci only SUVH2 is required, and this locus specificity correlates with the differing DNA-binding affinity of the SRA domains within SUVH9 and SUVH2. Specifically, SUVH9 preferentially binds methylated asymmetric sites, while SUVH2 preferentially binds methylated CG sites. The suvh9 and suvh2 mutations do not eliminate siRNAs, suggesting a role for SUVH9 and SUVH2 late in the RNA-directed DNA methylation pathway. With these new results, it is clear that SRA-domain proteins are involved in each of the three pathways leading to DNA methylation in Arabidopsis. Public Library of Science 2008-11-28 /pmc/articles/PMC2582956/ /pubmed/19043555 http://dx.doi.org/10.1371/journal.pgen.1000280 Text en Johnson et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Johnson, Lianna M. Law, Julie A. Khattar, Anuj Henderson, Ian R. Jacobsen, Steven E. SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation |
title | SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation |
title_full | SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation |
title_fullStr | SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation |
title_full_unstemmed | SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation |
title_short | SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation |
title_sort | sra-domain proteins required for drm2-mediated de novo dna methylation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582956/ https://www.ncbi.nlm.nih.gov/pubmed/19043555 http://dx.doi.org/10.1371/journal.pgen.1000280 |
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