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SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation

De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Her...

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Detalles Bibliográficos
Autores principales: Johnson, Lianna M., Law, Julie A., Khattar, Anuj, Henderson, Ian R., Jacobsen, Steven E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582956/
https://www.ncbi.nlm.nih.gov/pubmed/19043555
http://dx.doi.org/10.1371/journal.pgen.1000280
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author Johnson, Lianna M.
Law, Julie A.
Khattar, Anuj
Henderson, Ian R.
Jacobsen, Steven E.
author_facet Johnson, Lianna M.
Law, Julie A.
Khattar, Anuj
Henderson, Ian R.
Jacobsen, Steven E.
author_sort Johnson, Lianna M.
collection PubMed
description De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Here, we show that two SRA-domain proteins (SUVH9 and SUVH2) are also essential for DRM2-mediated de novo and maintenance DNA methylation in Arabidopsis thaliana. At some loci, SUVH9 and SUVH2 act redundantly, while at other loci only SUVH2 is required, and this locus specificity correlates with the differing DNA-binding affinity of the SRA domains within SUVH9 and SUVH2. Specifically, SUVH9 preferentially binds methylated asymmetric sites, while SUVH2 preferentially binds methylated CG sites. The suvh9 and suvh2 mutations do not eliminate siRNAs, suggesting a role for SUVH9 and SUVH2 late in the RNA-directed DNA methylation pathway. With these new results, it is clear that SRA-domain proteins are involved in each of the three pathways leading to DNA methylation in Arabidopsis.
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spelling pubmed-25829562008-11-28 SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation Johnson, Lianna M. Law, Julie A. Khattar, Anuj Henderson, Ian R. Jacobsen, Steven E. PLoS Genet Research Article De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Here, we show that two SRA-domain proteins (SUVH9 and SUVH2) are also essential for DRM2-mediated de novo and maintenance DNA methylation in Arabidopsis thaliana. At some loci, SUVH9 and SUVH2 act redundantly, while at other loci only SUVH2 is required, and this locus specificity correlates with the differing DNA-binding affinity of the SRA domains within SUVH9 and SUVH2. Specifically, SUVH9 preferentially binds methylated asymmetric sites, while SUVH2 preferentially binds methylated CG sites. The suvh9 and suvh2 mutations do not eliminate siRNAs, suggesting a role for SUVH9 and SUVH2 late in the RNA-directed DNA methylation pathway. With these new results, it is clear that SRA-domain proteins are involved in each of the three pathways leading to DNA methylation in Arabidopsis. Public Library of Science 2008-11-28 /pmc/articles/PMC2582956/ /pubmed/19043555 http://dx.doi.org/10.1371/journal.pgen.1000280 Text en Johnson et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Johnson, Lianna M.
Law, Julie A.
Khattar, Anuj
Henderson, Ian R.
Jacobsen, Steven E.
SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation
title SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation
title_full SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation
title_fullStr SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation
title_full_unstemmed SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation
title_short SRA-Domain Proteins Required for DRM2-Mediated De Novo DNA Methylation
title_sort sra-domain proteins required for drm2-mediated de novo dna methylation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582956/
https://www.ncbi.nlm.nih.gov/pubmed/19043555
http://dx.doi.org/10.1371/journal.pgen.1000280
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