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Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands
ATP in the 100 μM-1 mM concentration range provoked a calcium-independent increase of the oxidation of dichlorodihydrofluorescein (DCFH) to dichlorofluorescein (DCF) by mouse submandibular cells. 3′-O-(4-benzoyl)benzoyl adenosine 5′-triphosphate (BzATP), a P2X(7) agonist, but not a muscarinic or an...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer Netherlands
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583204/ https://www.ncbi.nlm.nih.gov/pubmed/18581262 http://dx.doi.org/10.1007/s11302-008-9118-y |
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author | Seil, Michèle Fontanils, Unai Etxebarria, Irantzu Gorrono Pochet, Stéphanie Garcia-Marcos, Mikel Marino, Aida Dehaye, Jean-Paul |
author_facet | Seil, Michèle Fontanils, Unai Etxebarria, Irantzu Gorrono Pochet, Stéphanie Garcia-Marcos, Mikel Marino, Aida Dehaye, Jean-Paul |
author_sort | Seil, Michèle |
collection | PubMed |
description | ATP in the 100 μM-1 mM concentration range provoked a calcium-independent increase of the oxidation of dichlorodihydrofluorescein (DCFH) to dichlorofluorescein (DCF) by mouse submandibular cells. 3′-O-(4-benzoyl)benzoyl adenosine 5′-triphosphate (BzATP), a P2X(7) agonist, but not a muscarinic or an adrenergic agonist, reproduced the effect of ATP. The inhibition of phospholipase C by U73122 or the potentiation of P2X(4) receptor activation with ivermectin did not modify the response to ATP. ATP did not increase the oxidation of DCFH in cells isolated from submandibular glands of P2X(7) knockout mice or in cells pretreated with a P2X(7) antagonist. The inhibition of protein kinase C or of mitogen-activated protein kinase (MAP kinase) or of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocked the oxidation of DCFH without affecting the increase of the intracellular concentration of calcium or the uptake of ethidium bromide in response to extracellular ATP. From these results it is concluded that the activation of the P2X(7) receptors from submandibular glands triggers an intracellular signalling cascade involving protein kinase C and MAP kinase leading to the stimulation of NADPH oxidase and the subsequent generation of reactive oxygen species. |
format | Text |
id | pubmed-2583204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-25832042009-01-26 Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands Seil, Michèle Fontanils, Unai Etxebarria, Irantzu Gorrono Pochet, Stéphanie Garcia-Marcos, Mikel Marino, Aida Dehaye, Jean-Paul Purinergic Signal Original Article ATP in the 100 μM-1 mM concentration range provoked a calcium-independent increase of the oxidation of dichlorodihydrofluorescein (DCFH) to dichlorofluorescein (DCF) by mouse submandibular cells. 3′-O-(4-benzoyl)benzoyl adenosine 5′-triphosphate (BzATP), a P2X(7) agonist, but not a muscarinic or an adrenergic agonist, reproduced the effect of ATP. The inhibition of phospholipase C by U73122 or the potentiation of P2X(4) receptor activation with ivermectin did not modify the response to ATP. ATP did not increase the oxidation of DCFH in cells isolated from submandibular glands of P2X(7) knockout mice or in cells pretreated with a P2X(7) antagonist. The inhibition of protein kinase C or of mitogen-activated protein kinase (MAP kinase) or of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocked the oxidation of DCFH without affecting the increase of the intracellular concentration of calcium or the uptake of ethidium bromide in response to extracellular ATP. From these results it is concluded that the activation of the P2X(7) receptors from submandibular glands triggers an intracellular signalling cascade involving protein kinase C and MAP kinase leading to the stimulation of NADPH oxidase and the subsequent generation of reactive oxygen species. Springer Netherlands 2008-06-26 2008-12 /pmc/articles/PMC2583204/ /pubmed/18581262 http://dx.doi.org/10.1007/s11302-008-9118-y Text en © Springer Science+Business Media B.V. 2008 |
spellingShingle | Original Article Seil, Michèle Fontanils, Unai Etxebarria, Irantzu Gorrono Pochet, Stéphanie Garcia-Marcos, Mikel Marino, Aida Dehaye, Jean-Paul Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands |
title | Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands |
title_full | Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands |
title_fullStr | Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands |
title_full_unstemmed | Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands |
title_short | Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands |
title_sort | pharmacological evidence for the stimulation of nadph oxidase by p2x(7) receptors in mouse submandibular glands |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583204/ https://www.ncbi.nlm.nih.gov/pubmed/18581262 http://dx.doi.org/10.1007/s11302-008-9118-y |
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