High-affinity IgE receptor-β chain expression in human mast cells

The high-affinity IgE receptor (FcεRI)-β gene is one of the atopy-associated genes, but its biological significance is largely unknown. In this study, we generated the anti-FcεRI-β chain antibody to clarify β-chain protein expression in human mast cells. The FcεRI-β antibody showed specific binding...

Descripción completa

Detalles Bibliográficos
Autores principales: Matsuda, Akira, Okayama, Yoshimichi, Ebihara, Nobuyuki, Yokoi, Norihiko, Gao, Peisong, Hamuro, Junji, Hopkin, Julian M., Kinoshita, Shigeru
Formato: Texto
Lenguaje:English
Publicado: Elsevier 2008
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583255/
https://www.ncbi.nlm.nih.gov/pubmed/18571665
http://dx.doi.org/10.1016/j.jim.2008.05.006
_version_ 1782160739124903936
author Matsuda, Akira
Okayama, Yoshimichi
Ebihara, Nobuyuki
Yokoi, Norihiko
Gao, Peisong
Hamuro, Junji
Hopkin, Julian M.
Kinoshita, Shigeru
author_facet Matsuda, Akira
Okayama, Yoshimichi
Ebihara, Nobuyuki
Yokoi, Norihiko
Gao, Peisong
Hamuro, Junji
Hopkin, Julian M.
Kinoshita, Shigeru
author_sort Matsuda, Akira
collection PubMed
description The high-affinity IgE receptor (FcεRI)-β gene is one of the atopy-associated genes, but its biological significance is largely unknown. In this study, we generated the anti-FcεRI-β chain antibody to clarify β-chain protein expression in human mast cells. The FcεRI-β antibody showed specific binding to a 27 kDa protein with Western blotting and membrane bound immunostaining using cultured mast cells. Monomeric IgE sensitization increased β-chain expression as well as mature α-chain expression in mast cells. Upregulation of β-chain expression with monomeric IgE treatment suggests possible roles of FcεRI-β protein as an atopy-related molecule.
format Text
id pubmed-2583255
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-25832552008-12-02 High-affinity IgE receptor-β chain expression in human mast cells Matsuda, Akira Okayama, Yoshimichi Ebihara, Nobuyuki Yokoi, Norihiko Gao, Peisong Hamuro, Junji Hopkin, Julian M. Kinoshita, Shigeru J Immunol Methods Research Paper The high-affinity IgE receptor (FcεRI)-β gene is one of the atopy-associated genes, but its biological significance is largely unknown. In this study, we generated the anti-FcεRI-β chain antibody to clarify β-chain protein expression in human mast cells. The FcεRI-β antibody showed specific binding to a 27 kDa protein with Western blotting and membrane bound immunostaining using cultured mast cells. Monomeric IgE sensitization increased β-chain expression as well as mature α-chain expression in mast cells. Upregulation of β-chain expression with monomeric IgE treatment suggests possible roles of FcεRI-β protein as an atopy-related molecule. Elsevier 2008-07-31 /pmc/articles/PMC2583255/ /pubmed/18571665 http://dx.doi.org/10.1016/j.jim.2008.05.006 Text en © 2008 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Research Paper
Matsuda, Akira
Okayama, Yoshimichi
Ebihara, Nobuyuki
Yokoi, Norihiko
Gao, Peisong
Hamuro, Junji
Hopkin, Julian M.
Kinoshita, Shigeru
High-affinity IgE receptor-β chain expression in human mast cells
title High-affinity IgE receptor-β chain expression in human mast cells
title_full High-affinity IgE receptor-β chain expression in human mast cells
title_fullStr High-affinity IgE receptor-β chain expression in human mast cells
title_full_unstemmed High-affinity IgE receptor-β chain expression in human mast cells
title_short High-affinity IgE receptor-β chain expression in human mast cells
title_sort high-affinity ige receptor-β chain expression in human mast cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583255/
https://www.ncbi.nlm.nih.gov/pubmed/18571665
http://dx.doi.org/10.1016/j.jim.2008.05.006
work_keys_str_mv AT matsudaakira highaffinityigereceptorbchainexpressioninhumanmastcells
AT okayamayoshimichi highaffinityigereceptorbchainexpressioninhumanmastcells
AT ebiharanobuyuki highaffinityigereceptorbchainexpressioninhumanmastcells
AT yokoinorihiko highaffinityigereceptorbchainexpressioninhumanmastcells
AT gaopeisong highaffinityigereceptorbchainexpressioninhumanmastcells
AT hamurojunji highaffinityigereceptorbchainexpressioninhumanmastcells
AT hopkinjulianm highaffinityigereceptorbchainexpressioninhumanmastcells
AT kinoshitashigeru highaffinityigereceptorbchainexpressioninhumanmastcells

Ejemplares similares