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Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis
BACKGROUND: The Wuschel related homeobox (WOX) family proteins are key regulators implicated in the determination of cell fate in plants by preventing cell differentiation. A recent WOX phylogeny, based on WOX homeodomains, showed that all of the Physcomitrella patens and Selaginella moellendorffii...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2584047/ https://www.ncbi.nlm.nih.gov/pubmed/18950478 http://dx.doi.org/10.1186/1471-2148-8-291 |
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author | Deveaux, Yves Toffano-Nioche, Claire Claisse, Gaelle Thareau, Vincent Morin, Halima Laufs, Patrick Moreau, Hervé Kreis, Martin Lecharny, Alain |
author_facet | Deveaux, Yves Toffano-Nioche, Claire Claisse, Gaelle Thareau, Vincent Morin, Halima Laufs, Patrick Moreau, Hervé Kreis, Martin Lecharny, Alain |
author_sort | Deveaux, Yves |
collection | PubMed |
description | BACKGROUND: The Wuschel related homeobox (WOX) family proteins are key regulators implicated in the determination of cell fate in plants by preventing cell differentiation. A recent WOX phylogeny, based on WOX homeodomains, showed that all of the Physcomitrella patens and Selaginella moellendorffii WOX proteins clustered into a single orthologous group. We hypothesized that members of this group might preferentially share a significant part of their function in phylogenetically distant organisms. Hence, we first validated the limits of the WOX13 orthologous group (WOX13 OG) using the occurrence of other clade specific signatures and conserved intron insertion sites. Secondly, a functional analysis using expression data and mutants was undertaken. RESULTS: The WOX13 OG contained the most conserved plant WOX proteins including the only WOX detected in the highly proliferating basal unicellular and photosynthetic organism Ostreococcus tauri. A large expansion of the WOX family was observed after the separation of mosses from other land plants and before monocots and dicots have arisen. In Arabidopsis thaliana, AtWOX13 was dynamically expressed during primary and lateral root initiation and development, in gynoecium and during embryo development. AtWOX13 appeared to affect the floral transition. An intriguing clade, represented by the functional AtWOX14 gene inside the WOX13 OG, was only found in the Brassicaceae. Compared to AtWOX13, the gene expression profile of AtWOX14 was restricted to the early stages of lateral root formation and specific to developing anthers. A mutational insertion upstream of the AtWOX14 homeodomain sequence led to abnormal root development, a delay in the floral transition and premature anther differentiation. CONCLUSION: Our data provide evidence in favor of the WOX13 OG as the clade containing the most conserved WOX genes and established a functional link to organ initiation and development in Arabidopsis, most likely by preventing premature differentiation. The future use of Ostreococcus tauri and Physcomitrella patens as biological models should allow us to obtain a better insight into the functional importance of WOX13 OG genes. |
format | Text |
id | pubmed-2584047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-25840472008-11-18 Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis Deveaux, Yves Toffano-Nioche, Claire Claisse, Gaelle Thareau, Vincent Morin, Halima Laufs, Patrick Moreau, Hervé Kreis, Martin Lecharny, Alain BMC Evol Biol Research Article BACKGROUND: The Wuschel related homeobox (WOX) family proteins are key regulators implicated in the determination of cell fate in plants by preventing cell differentiation. A recent WOX phylogeny, based on WOX homeodomains, showed that all of the Physcomitrella patens and Selaginella moellendorffii WOX proteins clustered into a single orthologous group. We hypothesized that members of this group might preferentially share a significant part of their function in phylogenetically distant organisms. Hence, we first validated the limits of the WOX13 orthologous group (WOX13 OG) using the occurrence of other clade specific signatures and conserved intron insertion sites. Secondly, a functional analysis using expression data and mutants was undertaken. RESULTS: The WOX13 OG contained the most conserved plant WOX proteins including the only WOX detected in the highly proliferating basal unicellular and photosynthetic organism Ostreococcus tauri. A large expansion of the WOX family was observed after the separation of mosses from other land plants and before monocots and dicots have arisen. In Arabidopsis thaliana, AtWOX13 was dynamically expressed during primary and lateral root initiation and development, in gynoecium and during embryo development. AtWOX13 appeared to affect the floral transition. An intriguing clade, represented by the functional AtWOX14 gene inside the WOX13 OG, was only found in the Brassicaceae. Compared to AtWOX13, the gene expression profile of AtWOX14 was restricted to the early stages of lateral root formation and specific to developing anthers. A mutational insertion upstream of the AtWOX14 homeodomain sequence led to abnormal root development, a delay in the floral transition and premature anther differentiation. CONCLUSION: Our data provide evidence in favor of the WOX13 OG as the clade containing the most conserved WOX genes and established a functional link to organ initiation and development in Arabidopsis, most likely by preventing premature differentiation. The future use of Ostreococcus tauri and Physcomitrella patens as biological models should allow us to obtain a better insight into the functional importance of WOX13 OG genes. BioMed Central 2008-10-24 /pmc/articles/PMC2584047/ /pubmed/18950478 http://dx.doi.org/10.1186/1471-2148-8-291 Text en Copyright ©2008 Deveaux et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Deveaux, Yves Toffano-Nioche, Claire Claisse, Gaelle Thareau, Vincent Morin, Halima Laufs, Patrick Moreau, Hervé Kreis, Martin Lecharny, Alain Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis |
title | Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis |
title_full | Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis |
title_fullStr | Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis |
title_full_unstemmed | Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis |
title_short | Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis |
title_sort | genes of the most conserved wox clade in plants affect root and flower development in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2584047/ https://www.ncbi.nlm.nih.gov/pubmed/18950478 http://dx.doi.org/10.1186/1471-2148-8-291 |
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