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Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
BACKGROUND: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketol...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2585806/ https://www.ncbi.nlm.nih.gov/pubmed/19052639 http://dx.doi.org/10.1371/journal.pone.0003847 |
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author | Panagiotou, Gianni Andersen, Mikael R. Grotkjær, Thomas Regueira, Torsten B. Hofmann, Gerald Nielsen, Jens Olsson, Lisbeth |
author_facet | Panagiotou, Gianni Andersen, Mikael R. Grotkjær, Thomas Regueira, Torsten B. Hofmann, Gerald Nielsen, Jens Olsson, Lisbeth |
author_sort | Panagiotou, Gianni |
collection | PubMed |
description | BACKGROUND: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. METHODOLOGY/PRINCIPAL FINDINGS: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by over-expression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. CONCLUSIONS/SIGNIFICANCE: Metabolic flux analysis using (13)C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources. |
format | Text |
id | pubmed-2585806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-25858062008-12-04 Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans Panagiotou, Gianni Andersen, Mikael R. Grotkjær, Thomas Regueira, Torsten B. Hofmann, Gerald Nielsen, Jens Olsson, Lisbeth PLoS One Research Article BACKGROUND: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. METHODOLOGY/PRINCIPAL FINDINGS: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by over-expression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. CONCLUSIONS/SIGNIFICANCE: Metabolic flux analysis using (13)C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources. Public Library of Science 2008-12-04 /pmc/articles/PMC2585806/ /pubmed/19052639 http://dx.doi.org/10.1371/journal.pone.0003847 Text en Panagiotou et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Panagiotou, Gianni Andersen, Mikael R. Grotkjær, Thomas Regueira, Torsten B. Hofmann, Gerald Nielsen, Jens Olsson, Lisbeth Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans |
title | Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
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title_full | Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
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title_fullStr | Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
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title_full_unstemmed | Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
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title_short | Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
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title_sort | systems analysis unfolds the relationship between the phosphoketolase pathway and growth in aspergillus nidulans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2585806/ https://www.ncbi.nlm.nih.gov/pubmed/19052639 http://dx.doi.org/10.1371/journal.pone.0003847 |
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