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Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans

BACKGROUND: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketol...

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Autores principales: Panagiotou, Gianni, Andersen, Mikael R., Grotkjær, Thomas, Regueira, Torsten B., Hofmann, Gerald, Nielsen, Jens, Olsson, Lisbeth
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2585806/
https://www.ncbi.nlm.nih.gov/pubmed/19052639
http://dx.doi.org/10.1371/journal.pone.0003847
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author Panagiotou, Gianni
Andersen, Mikael R.
Grotkjær, Thomas
Regueira, Torsten B.
Hofmann, Gerald
Nielsen, Jens
Olsson, Lisbeth
author_facet Panagiotou, Gianni
Andersen, Mikael R.
Grotkjær, Thomas
Regueira, Torsten B.
Hofmann, Gerald
Nielsen, Jens
Olsson, Lisbeth
author_sort Panagiotou, Gianni
collection PubMed
description BACKGROUND: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. METHODOLOGY/PRINCIPAL FINDINGS: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by over-expression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. CONCLUSIONS/SIGNIFICANCE: Metabolic flux analysis using (13)C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources.
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spelling pubmed-25858062008-12-04 Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans Panagiotou, Gianni Andersen, Mikael R. Grotkjær, Thomas Regueira, Torsten B. Hofmann, Gerald Nielsen, Jens Olsson, Lisbeth PLoS One Research Article BACKGROUND: Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity. METHODOLOGY/PRINCIPAL FINDINGS: Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by over-expression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed that the response to phk over-expression is targeted to neutralize the effect of the over-expression by regulating the acetate metabolism and initiate a growth dampening response. CONCLUSIONS/SIGNIFICANCE: Metabolic flux analysis using (13)C-labelled glucose, showed that over-expression of phosphoketolase added flexibility to the central metabolism. Our findings further suggests that A. nidulans is not optimized for growth on xylose, glycerol or ethanol as the sole carbon sources. Public Library of Science 2008-12-04 /pmc/articles/PMC2585806/ /pubmed/19052639 http://dx.doi.org/10.1371/journal.pone.0003847 Text en Panagiotou et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Panagiotou, Gianni
Andersen, Mikael R.
Grotkjær, Thomas
Regueira, Torsten B.
Hofmann, Gerald
Nielsen, Jens
Olsson, Lisbeth
Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
title Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
title_full Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
title_fullStr Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
title_full_unstemmed Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
title_short Systems Analysis Unfolds the Relationship between the Phosphoketolase Pathway and Growth in Aspergillus nidulans
title_sort systems analysis unfolds the relationship between the phosphoketolase pathway and growth in aspergillus nidulans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2585806/
https://www.ncbi.nlm.nih.gov/pubmed/19052639
http://dx.doi.org/10.1371/journal.pone.0003847
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