Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.

This paper presents the first automated system for simultaneously detecting human papilloma, herpes simplex, adenovirus, or cytomegalovirus viral antigens and gene sequences in standard formalin-fixed, paraffin-embedded tissue substrates and tissue culture. These viruses can be detected by colorimet...

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Detalles Bibliográficos
Autores principales: Montone, K. T., Brigati, D. J., Budgeon, L. R.
Formato: Texto
Lenguaje:English
Publicado: Yale Journal of Biology and Medicine 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2589225/
https://www.ncbi.nlm.nih.gov/pubmed/2773514
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author Montone, K. T.
Brigati, D. J.
Budgeon, L. R.
author_facet Montone, K. T.
Brigati, D. J.
Budgeon, L. R.
author_sort Montone, K. T.
collection PubMed
description This paper presents the first automated system for simultaneously detecting human papilloma, herpes simplex, adenovirus, or cytomegalovirus viral antigens and gene sequences in standard formalin-fixed, paraffin-embedded tissue substrates and tissue culture. These viruses can be detected by colorimetric in situ nucleic acid hybridization, using biotinylated DNA probes, or by indirect immunoperoxidase techniques, using polyclonal or monoclonal antibodies, in a 2.0-hour assay performed at a single automated robotic workstation.
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spelling pubmed-25892252008-11-28 Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques. Montone, K. T. Brigati, D. J. Budgeon, L. R. Yale J Biol Med Research Article This paper presents the first automated system for simultaneously detecting human papilloma, herpes simplex, adenovirus, or cytomegalovirus viral antigens and gene sequences in standard formalin-fixed, paraffin-embedded tissue substrates and tissue culture. These viruses can be detected by colorimetric in situ nucleic acid hybridization, using biotinylated DNA probes, or by indirect immunoperoxidase techniques, using polyclonal or monoclonal antibodies, in a 2.0-hour assay performed at a single automated robotic workstation. Yale Journal of Biology and Medicine 1989 /pmc/articles/PMC2589225/ /pubmed/2773514 Text en
spellingShingle Research Article
Montone, K. T.
Brigati, D. J.
Budgeon, L. R.
Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.
title Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.
title_full Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.
title_fullStr Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.
title_full_unstemmed Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.
title_short Anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of DNA viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.
title_sort anatomic viral detection is automated: the application of a robotic molecular pathology system for the detection of dna viruses in anatomic pathology substrates, using immunocytochemical and nucleic acid hybridization techniques.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2589225/
https://www.ncbi.nlm.nih.gov/pubmed/2773514
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