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Electrogenicity of Na(+)-coupled bile acid transporters.

The Na(+)-bile acid cotransporters NTCP and ASBT are largely responsible for the Na(+)-dependent bile acid uptake in hepatocytes and intestinal epithelial cells, respectively. This review discusses the experimental methods available for demonstrating electrogenicity and examines the accumulating evi...

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Detalles Bibliográficos
Autor principal: Weinman, S. A.
Formato: Texto
Lenguaje:English
Publicado: Yale Journal of Biology and Medicine 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2589348/
https://www.ncbi.nlm.nih.gov/pubmed/9626753
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author Weinman, S. A.
author_facet Weinman, S. A.
author_sort Weinman, S. A.
collection PubMed
description The Na(+)-bile acid cotransporters NTCP and ASBT are largely responsible for the Na(+)-dependent bile acid uptake in hepatocytes and intestinal epithelial cells, respectively. This review discusses the experimental methods available for demonstrating electrogenicity and examines the accumulating evidence that coupled transport by each of these bile acid transporters is electrogenic. The evidence includes measurements of transport-associated currents by patch clamp electrophysiological techniques, as well as direct measurement of fluorescent bile acid transport rates in whole cell patch clamped, voltage clamped cells. The results support a Na+:bile acid coupling stoichiometry of 2:1.
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spelling pubmed-25893482008-12-01 Electrogenicity of Na(+)-coupled bile acid transporters. Weinman, S. A. Yale J Biol Med Research Article The Na(+)-bile acid cotransporters NTCP and ASBT are largely responsible for the Na(+)-dependent bile acid uptake in hepatocytes and intestinal epithelial cells, respectively. This review discusses the experimental methods available for demonstrating electrogenicity and examines the accumulating evidence that coupled transport by each of these bile acid transporters is electrogenic. The evidence includes measurements of transport-associated currents by patch clamp electrophysiological techniques, as well as direct measurement of fluorescent bile acid transport rates in whole cell patch clamped, voltage clamped cells. The results support a Na+:bile acid coupling stoichiometry of 2:1. Yale Journal of Biology and Medicine 1997 /pmc/articles/PMC2589348/ /pubmed/9626753 Text en
spellingShingle Research Article
Weinman, S. A.
Electrogenicity of Na(+)-coupled bile acid transporters.
title Electrogenicity of Na(+)-coupled bile acid transporters.
title_full Electrogenicity of Na(+)-coupled bile acid transporters.
title_fullStr Electrogenicity of Na(+)-coupled bile acid transporters.
title_full_unstemmed Electrogenicity of Na(+)-coupled bile acid transporters.
title_short Electrogenicity of Na(+)-coupled bile acid transporters.
title_sort electrogenicity of na(+)-coupled bile acid transporters.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2589348/
https://www.ncbi.nlm.nih.gov/pubmed/9626753
work_keys_str_mv AT weinmansa electrogenicityofnacoupledbileacidtransporters