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Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.

Until the 1980s the diagnosis of specific etiologic agents of infectious diseases rested with their isolation in vitro and identification by analysis of their phenotypic characteristics. In the 1970s the concept of a microbial species evolved from phenotypic analysis to nucleic acid homology. Curren...

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Detalles Bibliográficos
Autor principal: Edberg, S. C.
Formato: Texto
Lenguaje:English
Publicado: Yale Journal of Biology and Medicine 1985
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2589955/
https://www.ncbi.nlm.nih.gov/pubmed/3004048
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author Edberg, S. C.
author_facet Edberg, S. C.
author_sort Edberg, S. C.
collection PubMed
description Until the 1980s the diagnosis of specific etiologic agents of infectious diseases rested with their isolation in vitro and identification by analysis of their phenotypic characteristics. In the 1970s the concept of a microbial species evolved from phenotypic analysis to nucleic acid homology. Currently, nucleic acid sequences specific for a given species are being isolated and amplified and utilized not only to identify the pathogen after it has been grown in vitro but also elucidate it directly in biological material. The procedures for making nucleic acid hybridization probes are analogous to the generation of monoclonal antibody tests. Currently, research and development are centered in choosing the particular nucleic acid to analyze, establishing the most efficient vector system for amplifying the nucleic acid, generating an efficient means of selecting the particular nucleic acid fragment specific for the microorganism, and in measuring the hybridization reaction. While immunological techniques have been utilized in the clinical laboratory for over thirty years, the means of detecting nucleic acid hybridization reactions are just beginning to be usable in the clinical diagnostic laboratory. Much of nucleic acid hybridization research is proprietary, and a particular challenge is to develop a means whereby information can be used for the progress of science as a whole when generated by private ownership.
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spelling pubmed-25899552008-11-28 Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases. Edberg, S. C. Yale J Biol Med Research Article Until the 1980s the diagnosis of specific etiologic agents of infectious diseases rested with their isolation in vitro and identification by analysis of their phenotypic characteristics. In the 1970s the concept of a microbial species evolved from phenotypic analysis to nucleic acid homology. Currently, nucleic acid sequences specific for a given species are being isolated and amplified and utilized not only to identify the pathogen after it has been grown in vitro but also elucidate it directly in biological material. The procedures for making nucleic acid hybridization probes are analogous to the generation of monoclonal antibody tests. Currently, research and development are centered in choosing the particular nucleic acid to analyze, establishing the most efficient vector system for amplifying the nucleic acid, generating an efficient means of selecting the particular nucleic acid fragment specific for the microorganism, and in measuring the hybridization reaction. While immunological techniques have been utilized in the clinical laboratory for over thirty years, the means of detecting nucleic acid hybridization reactions are just beginning to be usable in the clinical diagnostic laboratory. Much of nucleic acid hybridization research is proprietary, and a particular challenge is to develop a means whereby information can be used for the progress of science as a whole when generated by private ownership. Yale Journal of Biology and Medicine 1985 /pmc/articles/PMC2589955/ /pubmed/3004048 Text en
spellingShingle Research Article
Edberg, S. C.
Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.
title Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.
title_full Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.
title_fullStr Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.
title_full_unstemmed Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.
title_short Principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.
title_sort principles of nucleic acid hybridization and comparison with monoclonal antibody technology for the diagnosis of infectious diseases.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2589955/
https://www.ncbi.nlm.nih.gov/pubmed/3004048
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