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The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene.

The spontaneous transfer of drug resistance genes has been shown to take place between cultured mammalian NIH-3T3 cells and occurs with a hierarchy of transfer efficiencies, transformed cells being more efficient than non-transformed cells. This experiment was accomplished by co-cultivating two NIH-...

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Detalles Bibliográficos
Autores principales: Wong, D., Liu, E., Cadman, E.
Formato: Texto
Lenguaje:English
Publicado: Yale Journal of Biology and Medicine 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2590405/
https://www.ncbi.nlm.nih.gov/pubmed/3284209
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author Wong, D.
Liu, E.
Cadman, E.
author_facet Wong, D.
Liu, E.
Cadman, E.
author_sort Wong, D.
collection PubMed
description The spontaneous transfer of drug resistance genes has been shown to take place between cultured mammalian NIH-3T3 cells and occurs with a hierarchy of transfer efficiencies, transformed cells being more efficient than non-transformed cells. This experiment was accomplished by co-cultivating two NIH-3T3 sublines, each transfected by standard plasmid methods with a different drug resistance gene, subjecting the mixed population to double selection by adding both drugs to the mixed cell culture, and isolating single cells which were resistant to both drugs. The genes used were the neo gene and gpt gene which conferred resistance to the drugs G418 and mycophenolic acid, respectively. DNA analysis confirmed the presence of both resistance genes in the cells which were resistant to both drugs. The mechanism of this gene transfer was by cell fusion rather than by chromosomal DNA uptake. The efficiency of gene transfer, as indicated by the number of double-resistant colonies standardized by number of cells cultured, was much higher between two sublines of cells transformed by the EJras oncogene than between one transformed and one non-transformed subline, which in turn was higher than between two non-transformed sublines. The higher efficiency of gene transfer between the transformed cells also occurred when these cells were injected into nude mice, thus demonstrating that the same process occurred in vivo. It would appear that drug resistance genes may be transferred spontaneously in cultured mammalian cells by cell fusion, and that transformed cells have a higher efficiency of gene transfer compared to non-transformed cells.
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spelling pubmed-25904052008-11-28 The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene. Wong, D. Liu, E. Cadman, E. Yale J Biol Med Research Article The spontaneous transfer of drug resistance genes has been shown to take place between cultured mammalian NIH-3T3 cells and occurs with a hierarchy of transfer efficiencies, transformed cells being more efficient than non-transformed cells. This experiment was accomplished by co-cultivating two NIH-3T3 sublines, each transfected by standard plasmid methods with a different drug resistance gene, subjecting the mixed population to double selection by adding both drugs to the mixed cell culture, and isolating single cells which were resistant to both drugs. The genes used were the neo gene and gpt gene which conferred resistance to the drugs G418 and mycophenolic acid, respectively. DNA analysis confirmed the presence of both resistance genes in the cells which were resistant to both drugs. The mechanism of this gene transfer was by cell fusion rather than by chromosomal DNA uptake. The efficiency of gene transfer, as indicated by the number of double-resistant colonies standardized by number of cells cultured, was much higher between two sublines of cells transformed by the EJras oncogene than between one transformed and one non-transformed subline, which in turn was higher than between two non-transformed sublines. The higher efficiency of gene transfer between the transformed cells also occurred when these cells were injected into nude mice, thus demonstrating that the same process occurred in vivo. It would appear that drug resistance genes may be transferred spontaneously in cultured mammalian cells by cell fusion, and that transformed cells have a higher efficiency of gene transfer compared to non-transformed cells. Yale Journal of Biology and Medicine 1988 /pmc/articles/PMC2590405/ /pubmed/3284209 Text en
spellingShingle Research Article
Wong, D.
Liu, E.
Cadman, E.
The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene.
title The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene.
title_full The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene.
title_fullStr The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene.
title_full_unstemmed The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene.
title_short The enhanced transfer of drug-resistant genes in NIH-3T3 cells transformed by the EJras oncogene.
title_sort enhanced transfer of drug-resistant genes in nih-3t3 cells transformed by the ejras oncogene.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2590405/
https://www.ncbi.nlm.nih.gov/pubmed/3284209
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