Cargando…
Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas.
The osmotic stability of M. gallisepticum was found to be a consequence of the synthesis of disaturated phosphatidylcholine incorporated into the cell membrane. The disaturated lipid induces the formation of segregated lipid domains, thus providing the sites for increased permeation of ions. Such pe...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Yale Journal of Biology and Medicine
1983
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2590543/ https://www.ncbi.nlm.nih.gov/pubmed/6089452 |
_version_ | 1782161335174299648 |
---|---|
author | Rottem, S. Shirvan, M. H. Gross, Z. |
author_facet | Rottem, S. Shirvan, M. H. Gross, Z. |
author_sort | Rottem, S. |
collection | PubMed |
description | The osmotic stability of M. gallisepticum was found to be a consequence of the synthesis of disaturated phosphatidylcholine incorporated into the cell membrane. The disaturated lipid induces the formation of segregated lipid domains, thus providing the sites for increased permeation of ions. Such permeation reduces the internal pressure so as to minimize cell swelling and subsequent lysis in a hypotonic medium. Purified membranes of M. gallisepticum can be prepared from cells suspended in an iso-osmotic NaCl solution containing either dicyclohexylcarbodiimide (DCCD), which blocks ATPase activity, or a mild alkaline buffer. Both conditions seem to interfere with cell volume regulation. These procedures can be used also to isolate membranes of other osmotically stable mycoplasmas. |
format | Text |
id | pubmed-2590543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
publisher | Yale Journal of Biology and Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-25905432008-11-28 Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. Rottem, S. Shirvan, M. H. Gross, Z. Yale J Biol Med Research Article The osmotic stability of M. gallisepticum was found to be a consequence of the synthesis of disaturated phosphatidylcholine incorporated into the cell membrane. The disaturated lipid induces the formation of segregated lipid domains, thus providing the sites for increased permeation of ions. Such permeation reduces the internal pressure so as to minimize cell swelling and subsequent lysis in a hypotonic medium. Purified membranes of M. gallisepticum can be prepared from cells suspended in an iso-osmotic NaCl solution containing either dicyclohexylcarbodiimide (DCCD), which blocks ATPase activity, or a mild alkaline buffer. Both conditions seem to interfere with cell volume regulation. These procedures can be used also to isolate membranes of other osmotically stable mycoplasmas. Yale Journal of Biology and Medicine 1983 /pmc/articles/PMC2590543/ /pubmed/6089452 Text en |
spellingShingle | Research Article Rottem, S. Shirvan, M. H. Gross, Z. Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. |
title | Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. |
title_full | Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. |
title_fullStr | Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. |
title_full_unstemmed | Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. |
title_short | Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. |
title_sort | phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2590543/ https://www.ncbi.nlm.nih.gov/pubmed/6089452 |
work_keys_str_mv | AT rottems phaseseparationionpermeabilityandtheisolationofmembranesfromosmoticallystablemycoplasmas AT shirvanmh phaseseparationionpermeabilityandtheisolationofmembranesfromosmoticallystablemycoplasmas AT grossz phaseseparationionpermeabilityandtheisolationofmembranesfromosmoticallystablemycoplasmas |