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Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells

NPM (nucleophosmin; also known as B23) is an abundantly and ubiquitously expressed multifunctional nucleolar phosphoprotein, which is involved in numerous cellular processes, including ribosome biogenesis, protein chaperoning and centrosome duplication; however, the role of NPM in the cell cycle sti...

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Autores principales: Amin, Mohammed Abdullahel, Matsunaga, Sachihiro, Uchiyama, Susumu, Fukui, Kiichi
Formato: Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2590675/
https://www.ncbi.nlm.nih.gov/pubmed/18729828
http://dx.doi.org/10.1042/BJ20081411
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author Amin, Mohammed Abdullahel
Matsunaga, Sachihiro
Uchiyama, Susumu
Fukui, Kiichi
author_facet Amin, Mohammed Abdullahel
Matsunaga, Sachihiro
Uchiyama, Susumu
Fukui, Kiichi
author_sort Amin, Mohammed Abdullahel
collection PubMed
description NPM (nucleophosmin; also known as B23) is an abundantly and ubiquitously expressed multifunctional nucleolar phosphoprotein, which is involved in numerous cellular processes, including ribosome biogenesis, protein chaperoning and centrosome duplication; however, the role of NPM in the cell cycle still remains unknown. In the present study, we show dynamic localization of NPM throughout the cell cycle of HeLa cells. Using a combination of RNAi (RNA interference) and three-dimensional microscopy we show that NPM is localized at the chromosome periphery during mitosis. We also demonstrate that depletion of NPM causes distortion of nucleolar structure as expected and leads to unexpected dramatic changes in nuclear morphology with multiple micronuclei formation. The defect in nuclear shape of NPM-depleted cells, which is clearly observed by live-cell imaging, is due to the distortion of cytoskeletal (α-tubulin and β-actin) structure, resulting from the defects in centrosomal microtubule nucleation. These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells.
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spelling pubmed-25906752008-12-03 Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells Amin, Mohammed Abdullahel Matsunaga, Sachihiro Uchiyama, Susumu Fukui, Kiichi Biochem J Accelerated Publication NPM (nucleophosmin; also known as B23) is an abundantly and ubiquitously expressed multifunctional nucleolar phosphoprotein, which is involved in numerous cellular processes, including ribosome biogenesis, protein chaperoning and centrosome duplication; however, the role of NPM in the cell cycle still remains unknown. In the present study, we show dynamic localization of NPM throughout the cell cycle of HeLa cells. Using a combination of RNAi (RNA interference) and three-dimensional microscopy we show that NPM is localized at the chromosome periphery during mitosis. We also demonstrate that depletion of NPM causes distortion of nucleolar structure as expected and leads to unexpected dramatic changes in nuclear morphology with multiple micronuclei formation. The defect in nuclear shape of NPM-depleted cells, which is clearly observed by live-cell imaging, is due to the distortion of cytoskeletal (α-tubulin and β-actin) structure, resulting from the defects in centrosomal microtubule nucleation. These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells. Portland Press Ltd. 2008-10-15 2008-11-01 /pmc/articles/PMC2590675/ /pubmed/18729828 http://dx.doi.org/10.1042/BJ20081411 Text en © 2008 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by-nc/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Accelerated Publication
Amin, Mohammed Abdullahel
Matsunaga, Sachihiro
Uchiyama, Susumu
Fukui, Kiichi
Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
title Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
title_full Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
title_fullStr Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
title_full_unstemmed Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
title_short Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
title_sort depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in hela cells
topic Accelerated Publication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2590675/
https://www.ncbi.nlm.nih.gov/pubmed/18729828
http://dx.doi.org/10.1042/BJ20081411
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