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Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells

It has been shown that testicular germ cell development is critically dependent upon somatic cell activity but, conversely, the extent to which germ cells normally regulate somatic cell function is less clear. This study was designed, therefore, to examine the effect of germ cell depletion on Sertol...

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Autores principales: J O'Shaughnessy, P, Hu, L, Baker, P J
Formato: Texto
Lenguaje:English
Publicado: BioScientifica 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2592074/
https://www.ncbi.nlm.nih.gov/pubmed/18390686
http://dx.doi.org/10.1530/REP-08-0012
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author J O'Shaughnessy, P
Hu, L
Baker, P J
author_facet J O'Shaughnessy, P
Hu, L
Baker, P J
author_sort J O'Shaughnessy, P
collection PubMed
description It has been shown that testicular germ cell development is critically dependent upon somatic cell activity but, conversely, the extent to which germ cells normally regulate somatic cell function is less clear. This study was designed, therefore, to examine the effect of germ cell depletion on Sertoli cell and Leydig cell transcript levels. Mice were treated with busulphan to deplete the germ cell population and levels of mRNA transcripts encoding 26 Sertoli cell-specific proteins and 6 Leydig cell proteins were measured by real-time PCR up to 50 days after treatment. Spermatogonia were lost from the testis between 5 and 10 days after treatment, while spermatocytes were depleted after 10 days and spermatids after 20 days. By 30 days after treatment, most tubules were devoid of germ cells. Circulating FSH and intratesticular testosterone were not significantly affected by treatment. Of the 26 Sertoli cell markers tested, 13 showed no change in transcript levels after busulphan treatment, 2 showed decreased levels, 9 showed increased levels and 2 showed a biphasic response. In 60% of cases, changes in transcript levels occurred after the loss of the spermatids. Levels of mRNA transcripts encoding Leydig cell-specific products related to steroidogenesis were unaffected by treatment. Results indicate (1) that germ cells play a major and widespread role in the regulation of Sertoli cell activity, (2) most changes in transcript levels are associated with the loss of spermatids and (3) Leydig cell steroidogenesis is largely unaffected by germ cell ablation.
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spelling pubmed-25920742008-12-01 Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells J O'Shaughnessy, P Hu, L Baker, P J Reproduction Research It has been shown that testicular germ cell development is critically dependent upon somatic cell activity but, conversely, the extent to which germ cells normally regulate somatic cell function is less clear. This study was designed, therefore, to examine the effect of germ cell depletion on Sertoli cell and Leydig cell transcript levels. Mice were treated with busulphan to deplete the germ cell population and levels of mRNA transcripts encoding 26 Sertoli cell-specific proteins and 6 Leydig cell proteins were measured by real-time PCR up to 50 days after treatment. Spermatogonia were lost from the testis between 5 and 10 days after treatment, while spermatocytes were depleted after 10 days and spermatids after 20 days. By 30 days after treatment, most tubules were devoid of germ cells. Circulating FSH and intratesticular testosterone were not significantly affected by treatment. Of the 26 Sertoli cell markers tested, 13 showed no change in transcript levels after busulphan treatment, 2 showed decreased levels, 9 showed increased levels and 2 showed a biphasic response. In 60% of cases, changes in transcript levels occurred after the loss of the spermatids. Levels of mRNA transcripts encoding Leydig cell-specific products related to steroidogenesis were unaffected by treatment. Results indicate (1) that germ cells play a major and widespread role in the regulation of Sertoli cell activity, (2) most changes in transcript levels are associated with the loss of spermatids and (3) Leydig cell steroidogenesis is largely unaffected by germ cell ablation. BioScientifica 2008-06 /pmc/articles/PMC2592074/ /pubmed/18390686 http://dx.doi.org/10.1530/REP-08-0012 Text en © 2008 Society for Reproduction and Fertility http://www.endocrinology.org/journals/reuselicence/ This is an Open Access article distributed under the terms of the Society for Reproduction and Fertility's Re-use Licence (http://www.endocrinology.org/journals/reuselicence/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
J O'Shaughnessy, P
Hu, L
Baker, P J
Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells
title Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells
title_full Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells
title_fullStr Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells
title_full_unstemmed Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells
title_short Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells
title_sort effect of germ cell depletion on levels of specific mrna transcripts in mouse sertoli cells and leydig cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2592074/
https://www.ncbi.nlm.nih.gov/pubmed/18390686
http://dx.doi.org/10.1530/REP-08-0012
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