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Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase

The MCM gene from the archaeon Halobacterium, with and without its intein, was cloned into an Escherichia coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the inte...

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Detalles Bibliográficos
Autores principales:	Sakakibara, Nozomi, Han, Mimi, Rollor, Claire R, Gilson, Rebecca C, Busch, Courtney, Heo, Gunyoung, Kelman, Zvi
Formato:	Texto
Lenguaje:	English
Publicado:	Bentham Science Publishers Ltd 2008
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2593048/ https://www.ncbi.nlm.nih.gov/pubmed/19088906 http://dx.doi.org/10.2174/1874285800802010013

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