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Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.

Most erythropoietin (Ep) preparations contain non-erythropoietin contaminants. The use of such hormone concentrates raises important questions regarding interpretations of results derived from in vivo and especially from in vitro studies. By sterilizing various Ep preparations with Nalgene, Millipor...

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Detalles Bibliográficos
Autores principales: Gallicchio, V. S., Murphy, M. J.
Formato: Texto
Lenguaje:English
Publicado: Yale Journal of Biology and Medicine 1981
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2595979/
https://www.ncbi.nlm.nih.gov/pubmed/7324503
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author Gallicchio, V. S.
Murphy, M. J.
author_facet Gallicchio, V. S.
Murphy, M. J.
author_sort Gallicchio, V. S.
collection PubMed
description Most erythropoietin (Ep) preparations contain non-erythropoietin contaminants. The use of such hormone concentrates raises important questions regarding interpretations of results derived from in vivo and especially from in vitro studies. By sterilizing various Ep preparations with Nalgene, Millipore, or Selas silver filtration, or even after conventional membrane dialysis, variable responses were noted when the Ep was assayed with mouse bone marrow cells in vitro (i.e. by stimulating the production of erythroid colonies from CFU-e and BFU-e) and in vivo (i.e., by using the exhypoxic, polycythemic mouse bioassay for Ep). The utility and limitations of such preparative procedures are discussed.
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spelling pubmed-25959792008-12-05 Effect of membrane dialysis and filtration-sterilization on erythropoietin activity. Gallicchio, V. S. Murphy, M. J. Yale J Biol Med Research Article Most erythropoietin (Ep) preparations contain non-erythropoietin contaminants. The use of such hormone concentrates raises important questions regarding interpretations of results derived from in vivo and especially from in vitro studies. By sterilizing various Ep preparations with Nalgene, Millipore, or Selas silver filtration, or even after conventional membrane dialysis, variable responses were noted when the Ep was assayed with mouse bone marrow cells in vitro (i.e. by stimulating the production of erythroid colonies from CFU-e and BFU-e) and in vivo (i.e., by using the exhypoxic, polycythemic mouse bioassay for Ep). The utility and limitations of such preparative procedures are discussed. Yale Journal of Biology and Medicine 1981 /pmc/articles/PMC2595979/ /pubmed/7324503 Text en
spellingShingle Research Article
Gallicchio, V. S.
Murphy, M. J.
Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
title Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
title_full Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
title_fullStr Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
title_full_unstemmed Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
title_short Effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
title_sort effect of membrane dialysis and filtration-sterilization on erythropoietin activity.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2595979/
https://www.ncbi.nlm.nih.gov/pubmed/7324503
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