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Backscattering particle immunoassays in wire-guide droplet manipulations

A simpler way for manipulating droplets on a flat surface was demonstrated, eliminating the complications in the existing methods of open-surface digital microfluidics. Programmed and motorized movements of 10 μL droplets were demonstrated using stepper motors and microcontrollers, including merging...

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Detalles Bibliográficos
Autores principales: Yoon, Jeong-Yeol, You, David J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2596077/
https://www.ncbi.nlm.nih.gov/pubmed/19014703
http://dx.doi.org/10.1186/1754-1611-2-15
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author Yoon, Jeong-Yeol
You, David J
author_facet Yoon, Jeong-Yeol
You, David J
author_sort Yoon, Jeong-Yeol
collection PubMed
description A simpler way for manipulating droplets on a flat surface was demonstrated, eliminating the complications in the existing methods of open-surface digital microfluidics. Programmed and motorized movements of 10 μL droplets were demonstrated using stepper motors and microcontrollers, including merging, complicated movement along the programmed path, and rapid mixing. Latex immunoagglutination assays for mouse immunoglobulin G, bovine viral diarrhea virus and Escherichia coli were demonstrated by merging two droplets on a superhydrophobic surface (contact angle = 155 ± 2°) and using subsequent back light scattering detection, with detection limits of 50 pg mL(-1), 2.5 TCID(50 )mL(-1 )and 85 CFU mL(-1), respectively, all significantly lower than the other immunoassay demonstrations in conventional microfluidics (~1 ng mL(-1 )for proteins, ~100 TCID(50 )mL(-1 )for viruses and ~100 CFU mL(-1 )for bacteria). Advantages of this system over conventional microfluidics or microwell plate assays include: (1) minimized biofouling and repeated use (>100 times) of a platform; (2) possibility of nanoliter droplet manipulation; (3) reprogrammability with a computer or a game pad interface.
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spelling pubmed-25960772008-12-05 Backscattering particle immunoassays in wire-guide droplet manipulations Yoon, Jeong-Yeol You, David J J Biol Eng Research A simpler way for manipulating droplets on a flat surface was demonstrated, eliminating the complications in the existing methods of open-surface digital microfluidics. Programmed and motorized movements of 10 μL droplets were demonstrated using stepper motors and microcontrollers, including merging, complicated movement along the programmed path, and rapid mixing. Latex immunoagglutination assays for mouse immunoglobulin G, bovine viral diarrhea virus and Escherichia coli were demonstrated by merging two droplets on a superhydrophobic surface (contact angle = 155 ± 2°) and using subsequent back light scattering detection, with detection limits of 50 pg mL(-1), 2.5 TCID(50 )mL(-1 )and 85 CFU mL(-1), respectively, all significantly lower than the other immunoassay demonstrations in conventional microfluidics (~1 ng mL(-1 )for proteins, ~100 TCID(50 )mL(-1 )for viruses and ~100 CFU mL(-1 )for bacteria). Advantages of this system over conventional microfluidics or microwell plate assays include: (1) minimized biofouling and repeated use (>100 times) of a platform; (2) possibility of nanoliter droplet manipulation; (3) reprogrammability with a computer or a game pad interface. BioMed Central 2008-11-17 /pmc/articles/PMC2596077/ /pubmed/19014703 http://dx.doi.org/10.1186/1754-1611-2-15 Text en Copyright © 2008 Yoon and You; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Yoon, Jeong-Yeol
You, David J
Backscattering particle immunoassays in wire-guide droplet manipulations
title Backscattering particle immunoassays in wire-guide droplet manipulations
title_full Backscattering particle immunoassays in wire-guide droplet manipulations
title_fullStr Backscattering particle immunoassays in wire-guide droplet manipulations
title_full_unstemmed Backscattering particle immunoassays in wire-guide droplet manipulations
title_short Backscattering particle immunoassays in wire-guide droplet manipulations
title_sort backscattering particle immunoassays in wire-guide droplet manipulations
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2596077/
https://www.ncbi.nlm.nih.gov/pubmed/19014703
http://dx.doi.org/10.1186/1754-1611-2-15
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