An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.

An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile...

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Detalles Bibliográficos
Autores principales: Hsu, J. F., Evans, A. S., Niederman, J. C., Cenabre, L. C.
Formato: Texto
Lenguaje:English
Publicado: Yale Journal of Biology and Medicine 1982
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2596542/
https://www.ncbi.nlm.nih.gov/pubmed/6763815
Descripción
Sumario:An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile antibody in the test serum with the erythrocytes. A single serum dilution yields quantitative results when read in a spectrophotometer. The ELISA test showed a sensitivity comparable with the immune adherence hemagglutination assay (IAHA) and other heterophile tests, good reproducibility, and high specificity.

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