An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.

An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile...

Descripción completa

Detalles Bibliográficos
Autores principales: Hsu, J. F., Evans, A. S., Niederman, J. C., Cenabre, L. C.
Formato: Texto
Lenguaje:English
Publicado: Yale Journal of Biology and Medicine 1982
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2596542/
https://www.ncbi.nlm.nih.gov/pubmed/6763815
_version_ 1782161860339957760
author Hsu, J. F.
Evans, A. S.
Niederman, J. C.
Cenabre, L. C.
author_facet Hsu, J. F.
Evans, A. S.
Niederman, J. C.
Cenabre, L. C.
author_sort Hsu, J. F.
collection PubMed
description An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile antibody in the test serum with the erythrocytes. A single serum dilution yields quantitative results when read in a spectrophotometer. The ELISA test showed a sensitivity comparable with the immune adherence hemagglutination assay (IAHA) and other heterophile tests, good reproducibility, and high specificity.
format Text
id pubmed-2596542
institution National Center for Biotechnology Information
language English
publishDate 1982
publisher Yale Journal of Biology and Medicine
record_format MEDLINE/PubMed
spelling pubmed-25965422008-12-05 An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody. Hsu, J. F. Evans, A. S. Niederman, J. C. Cenabre, L. C. Yale J Biol Med Research Article An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile antibody in the test serum with the erythrocytes. A single serum dilution yields quantitative results when read in a spectrophotometer. The ELISA test showed a sensitivity comparable with the immune adherence hemagglutination assay (IAHA) and other heterophile tests, good reproducibility, and high specificity. Yale Journal of Biology and Medicine 1982 /pmc/articles/PMC2596542/ /pubmed/6763815 Text en
spellingShingle Research Article
Hsu, J. F.
Evans, A. S.
Niederman, J. C.
Cenabre, L. C.
An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
title An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
title_full An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
title_fullStr An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
title_full_unstemmed An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
title_short An enzyme-linked immunosorbent assay (ELISA) for measurement of heterophile antibody.
title_sort enzyme-linked immunosorbent assay (elisa) for measurement of heterophile antibody.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2596542/
https://www.ncbi.nlm.nih.gov/pubmed/6763815
work_keys_str_mv AT hsujf anenzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody
AT evansas anenzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody
AT niedermanjc anenzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody
AT cenabrelc anenzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody
AT hsujf enzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody
AT evansas enzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody
AT niedermanjc enzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody
AT cenabrelc enzymelinkedimmunosorbentassayelisaformeasurementofheterophileantibody

Ejemplares similares