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Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study

BACKGROUND: Inhalative exposure to vanadium pentoxide (V(2)O(5)) causes lung cancer in rodents. OBJECTIVE: The aim of the study was to investigate the impact of V(2)O(5) on DNA stability in workers from a V(2)O(5) factory. METHODS: We determined DNA strand breaks in leukocytes of 52 workers and cont...

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Autores principales: Ehrlich, Veronika A., Nersesyan, Armen K., Hoelzl, Christine, Ferk, Franziska, Bichler, Julia, Valic, Eva, Schaffer, Andreas, Schulte-Hermann, Rolf, Fenech, Michael, Wagner, Karl-Heinz, Knasmüller, Siegfried
Formato: Texto
Lenguaje:English
Publicado: National Institute of Environmental Health Sciences 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2599764/
https://www.ncbi.nlm.nih.gov/pubmed/19079721
http://dx.doi.org/10.1289/ehp.11438
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author Ehrlich, Veronika A.
Nersesyan, Armen K.
Hoelzl, Christine
Ferk, Franziska
Bichler, Julia
Valic, Eva
Schaffer, Andreas
Schulte-Hermann, Rolf
Fenech, Michael
Wagner, Karl-Heinz
Knasmüller, Siegfried
author_facet Ehrlich, Veronika A.
Nersesyan, Armen K.
Hoelzl, Christine
Ferk, Franziska
Bichler, Julia
Valic, Eva
Schaffer, Andreas
Schulte-Hermann, Rolf
Fenech, Michael
Wagner, Karl-Heinz
Knasmüller, Siegfried
author_sort Ehrlich, Veronika A.
collection PubMed
description BACKGROUND: Inhalative exposure to vanadium pentoxide (V(2)O(5)) causes lung cancer in rodents. OBJECTIVE: The aim of the study was to investigate the impact of V(2)O(5) on DNA stability in workers from a V(2)O(5) factory. METHODS: We determined DNA strand breaks in leukocytes of 52 workers and controls using the alkaline comet assay. We also investigated different parameters of chromosomal instability in lymphocytes of 23 workers and 24 controls using the cytokinesis-block micronucleus (MN) cytome method. RESULTS: Seven of eight biomarkers were increased in blood cells of the workers, and vanadium plasma concentrations in plasma were 7-fold higher than in the controls (0.31 μg/L). We observed no difference in DNA migration under standard conditions, but we found increased tail lengths due to formation of oxidized purines (7%) and pyrimidines (30%) with lesion-specific enzymes (formamidopyrimidine glycosylase and endonuclease III) in the workers. Bleomycin-induced DNA migration was higher in the exposed group (25%), whereas the repair of bleomycin-induced lesions was reduced. Workers had a 2.5-fold higher MN frequency, and nucleoplasmic bridges (NPBs) and nuclear buds (Nbuds) were increased 7-fold and 3-fold, respectively. Also, apoptosis and necrosis rates were higher, but only the latter parameter reached statistical significance. CONCLUSIONS: V(2)O(5) causes oxidation of DNA bases, affects DNA repair, and induces formation of MNs, NPBs, and Nbuds in blood cells, suggesting that the workers are at increased risk for cancer and other diseases that are related to DNA instability.
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spelling pubmed-25997642008-12-12 Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study Ehrlich, Veronika A. Nersesyan, Armen K. Hoelzl, Christine Ferk, Franziska Bichler, Julia Valic, Eva Schaffer, Andreas Schulte-Hermann, Rolf Fenech, Michael Wagner, Karl-Heinz Knasmüller, Siegfried Environ Health Perspect Research BACKGROUND: Inhalative exposure to vanadium pentoxide (V(2)O(5)) causes lung cancer in rodents. OBJECTIVE: The aim of the study was to investigate the impact of V(2)O(5) on DNA stability in workers from a V(2)O(5) factory. METHODS: We determined DNA strand breaks in leukocytes of 52 workers and controls using the alkaline comet assay. We also investigated different parameters of chromosomal instability in lymphocytes of 23 workers and 24 controls using the cytokinesis-block micronucleus (MN) cytome method. RESULTS: Seven of eight biomarkers were increased in blood cells of the workers, and vanadium plasma concentrations in plasma were 7-fold higher than in the controls (0.31 μg/L). We observed no difference in DNA migration under standard conditions, but we found increased tail lengths due to formation of oxidized purines (7%) and pyrimidines (30%) with lesion-specific enzymes (formamidopyrimidine glycosylase and endonuclease III) in the workers. Bleomycin-induced DNA migration was higher in the exposed group (25%), whereas the repair of bleomycin-induced lesions was reduced. Workers had a 2.5-fold higher MN frequency, and nucleoplasmic bridges (NPBs) and nuclear buds (Nbuds) were increased 7-fold and 3-fold, respectively. Also, apoptosis and necrosis rates were higher, but only the latter parameter reached statistical significance. CONCLUSIONS: V(2)O(5) causes oxidation of DNA bases, affects DNA repair, and induces formation of MNs, NPBs, and Nbuds in blood cells, suggesting that the workers are at increased risk for cancer and other diseases that are related to DNA instability. National Institute of Environmental Health Sciences 2008-12 2008-07-31 /pmc/articles/PMC2599764/ /pubmed/19079721 http://dx.doi.org/10.1289/ehp.11438 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, ?Reproduced with permission from Environmental Health Perspectives?); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.
spellingShingle Research
Ehrlich, Veronika A.
Nersesyan, Armen K.
Hoelzl, Christine
Ferk, Franziska
Bichler, Julia
Valic, Eva
Schaffer, Andreas
Schulte-Hermann, Rolf
Fenech, Michael
Wagner, Karl-Heinz
Knasmüller, Siegfried
Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study
title Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study
title_full Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study
title_fullStr Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study
title_full_unstemmed Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study
title_short Inhalative Exposure to Vanadium Pentoxide Causes DNA Damage in Workers: Results of a Multiple End Point Study
title_sort inhalative exposure to vanadium pentoxide causes dna damage in workers: results of a multiple end point study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2599764/
https://www.ncbi.nlm.nih.gov/pubmed/19079721
http://dx.doi.org/10.1289/ehp.11438
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