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High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability
High-level microsatellite instability (MSI-H) in colorectal cancer accounts for about 12% of colorectal cancers and is typically associated with a dense infiltration with cytotoxic CD8-positive lymphocytes. The role of regulatory T cells that may interfere with the host's antitumoural immune re...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2600708/ https://www.ncbi.nlm.nih.gov/pubmed/18985040 http://dx.doi.org/10.1038/sj.bjc.6604756 |
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author | Michel, S Benner, A Tariverdian, M Wentzensen, N Hoefler, P Pommerencke, T Grabe, N von Knebel Doeberitz, M Kloor, M |
author_facet | Michel, S Benner, A Tariverdian, M Wentzensen, N Hoefler, P Pommerencke, T Grabe, N von Knebel Doeberitz, M Kloor, M |
author_sort | Michel, S |
collection | PubMed |
description | High-level microsatellite instability (MSI-H) in colorectal cancer accounts for about 12% of colorectal cancers and is typically associated with a dense infiltration with cytotoxic CD8-positive lymphocytes. The role of regulatory T cells that may interfere with the host's antitumoural immune response in MSI-H colorectal cancers has not been analysed yet. Using an antibody directed against the regulatory T-cell marker transcription factor forkhead box P3 (FOXP3), regulatory T cells were examined in 70 colorectal cancers with known MSI status (MSI-H, n=37; microsatellite stable, n=33). In MSI-H colorectal cancers, we found a significantly higher intraepithelial infiltration with FOXP3-positive cells (median: 8.5 cells per 0.25 mm(2) vs 3.1 cells per 0.25 mm(2) in microsatellite stable, P<0.001), and a significantly elevated ratio of intraepithelial to stromal infiltration (0.05 vs 0.01 in microsatellite stable, P<0.001). CD8-positive cell counts were related positively to the number of FOXP3-positive cells (Spearman's ρ=0.56 and 0.55, respectively). Our results show that the elevated number of CD8-positive lymphocytes found in MSI-H colorectal cancers is paralleled by an enhanced infiltration with CD8-negative FOXP3-positive cells. These data suggest that FOXP3-positive cells may play a role in the regulation of the immune response directed against MSI-H colorectal cancers at the primary tumour site. |
format | Text |
id | pubmed-2600708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-26007082009-12-03 High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability Michel, S Benner, A Tariverdian, M Wentzensen, N Hoefler, P Pommerencke, T Grabe, N von Knebel Doeberitz, M Kloor, M Br J Cancer Molecular Diagnostics High-level microsatellite instability (MSI-H) in colorectal cancer accounts for about 12% of colorectal cancers and is typically associated with a dense infiltration with cytotoxic CD8-positive lymphocytes. The role of regulatory T cells that may interfere with the host's antitumoural immune response in MSI-H colorectal cancers has not been analysed yet. Using an antibody directed against the regulatory T-cell marker transcription factor forkhead box P3 (FOXP3), regulatory T cells were examined in 70 colorectal cancers with known MSI status (MSI-H, n=37; microsatellite stable, n=33). In MSI-H colorectal cancers, we found a significantly higher intraepithelial infiltration with FOXP3-positive cells (median: 8.5 cells per 0.25 mm(2) vs 3.1 cells per 0.25 mm(2) in microsatellite stable, P<0.001), and a significantly elevated ratio of intraepithelial to stromal infiltration (0.05 vs 0.01 in microsatellite stable, P<0.001). CD8-positive cell counts were related positively to the number of FOXP3-positive cells (Spearman's ρ=0.56 and 0.55, respectively). Our results show that the elevated number of CD8-positive lymphocytes found in MSI-H colorectal cancers is paralleled by an enhanced infiltration with CD8-negative FOXP3-positive cells. These data suggest that FOXP3-positive cells may play a role in the regulation of the immune response directed against MSI-H colorectal cancers at the primary tumour site. Nature Publishing Group 2008-12-02 2008-11-04 /pmc/articles/PMC2600708/ /pubmed/18985040 http://dx.doi.org/10.1038/sj.bjc.6604756 Text en Copyright © 2008 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular Diagnostics Michel, S Benner, A Tariverdian, M Wentzensen, N Hoefler, P Pommerencke, T Grabe, N von Knebel Doeberitz, M Kloor, M High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability |
title | High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability |
title_full | High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability |
title_fullStr | High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability |
title_full_unstemmed | High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability |
title_short | High density of FOXP3-positive T cells infiltrating colorectal cancers with microsatellite instability |
title_sort | high density of foxp3-positive t cells infiltrating colorectal cancers with microsatellite instability |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2600708/ https://www.ncbi.nlm.nih.gov/pubmed/18985040 http://dx.doi.org/10.1038/sj.bjc.6604756 |
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