Thermodynamic stability and Watson–Crick base pairing in the seed duplex are major determinants of the efficiency of the siRNA-based off-target effect

Short interfering RNA (siRNA) may down-regulate many unintended genes whose transcripts possess complementarity to the siRNA seed region, which contains 7 nt. The capability of siRNA to induce this off-target effect was highly correlated with the calculated melting temperature or standard free-energ...

Descripción completa

Detalles Bibliográficos
Autores principales: Ui-Tei, Kumiko, Naito, Yuki, Nishi, Kenji, Juni, Aya, Saigo, Kaoru
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602766/
https://www.ncbi.nlm.nih.gov/pubmed/18988625
http://dx.doi.org/10.1093/nar/gkn902

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602766/
https://www.ncbi.nlm.nih.gov/pubmed/18988625
http://dx.doi.org/10.1093/nar/gkn902

Ejemplares similares