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Errors in the bisulfite conversion of DNA: modulating inappropriate- and failed-conversion frequencies

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Bisulfite treatment can be used to ascertain the methylation states of individual cytosines in DNA. Ideally, bisulfite treatment deaminates unmethylated cytosines to uracils, and leaves 5-methylcytosines unchanged. Two types of bisulfite-conversion error occur: inappropriate conversion of 5-methylcy...

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Detalles Bibliográficos
Autores principales:	Genereux, Diane P., Johnson, Winslow C., Burden, Alice F., Stöger, Reinhard, Laird, Charles D.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2008
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2602783/ https://www.ncbi.nlm.nih.gov/pubmed/18984622 http://dx.doi.org/10.1093/nar/gkn691

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