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In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta
Herpesvirus infection of placenta may be harmful in pregnancy leading to disorders in fetal growth, premature delivery, miscarriage, or major congenital abnormalities. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been suggested...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2603597/ https://www.ncbi.nlm.nih.gov/pubmed/19115001 http://dx.doi.org/10.1371/journal.pone.0004073 |
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author | Di Stefano, Mariantonietta Calabrò, Maria Luisa Di Gangi, Iole Maria Cantatore, Santina Barbierato, Massimo Bergamo, Elisa Kfutwah, Anfumbom Jude Neri, Margherita Chieco-Bianchi, Luigi Greco, Pantaleo Gesualdo, Loreto Ayouba, Ahidjo Menu, Elisabeth Fiore, Josè Ramòn |
author_facet | Di Stefano, Mariantonietta Calabrò, Maria Luisa Di Gangi, Iole Maria Cantatore, Santina Barbierato, Massimo Bergamo, Elisa Kfutwah, Anfumbom Jude Neri, Margherita Chieco-Bianchi, Luigi Greco, Pantaleo Gesualdo, Loreto Ayouba, Ahidjo Menu, Elisabeth Fiore, Josè Ramòn |
author_sort | Di Stefano, Mariantonietta |
collection | PubMed |
description | Herpesvirus infection of placenta may be harmful in pregnancy leading to disorders in fetal growth, premature delivery, miscarriage, or major congenital abnormalities. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been suggested, and rare cases of in utero or perinatal HHV-8 transmission have been documented, no direct evidence of HHV-8 infection of placenta has yet been reported. The aim of this study was to evaluate the in vitro and in vivo susceptibility of placental cells to HHV-8 infection. Short-term infection assays were performed on placental chorionic villi isolated from term placentae. Qualitative and quantitative HHV-8 detection were performed by PCR and real-time PCR, and HHV-8 proteins were analyzed by immunohistochemistry. Term placenta samples from HHV-8-seropositive women were analyzed for the presence of HHV-8 DNA and antigens. In vitro infected histocultures showed increasing amounts of HHV-8 DNA in tissues and supernatants; cyto- and syncitiotrophoblasts, as well as endothelial cells, expressed latent and lytic viral antigens. Increased apoptotic phenomena were visualized by the terminal deoxynucleotidyl transferase-mediated deoxyuridine nick end-labeling method in infected histocultures. Ex vivo, HHV-8 DNA and a latent viral antigen were detected in placenta samples from HHV-8-seropositive women. These findings demonstrate that HHV-8, like other human herpesviruses, may infect placental cells in vitro and in vivo, thus providing evidence that this phenomenon might influence vertical transmission and pregnancy outcome in HHV-8-infected women. |
format | Text |
id | pubmed-2603597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26035972008-12-30 In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta Di Stefano, Mariantonietta Calabrò, Maria Luisa Di Gangi, Iole Maria Cantatore, Santina Barbierato, Massimo Bergamo, Elisa Kfutwah, Anfumbom Jude Neri, Margherita Chieco-Bianchi, Luigi Greco, Pantaleo Gesualdo, Loreto Ayouba, Ahidjo Menu, Elisabeth Fiore, Josè Ramòn PLoS One Research Article Herpesvirus infection of placenta may be harmful in pregnancy leading to disorders in fetal growth, premature delivery, miscarriage, or major congenital abnormalities. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been suggested, and rare cases of in utero or perinatal HHV-8 transmission have been documented, no direct evidence of HHV-8 infection of placenta has yet been reported. The aim of this study was to evaluate the in vitro and in vivo susceptibility of placental cells to HHV-8 infection. Short-term infection assays were performed on placental chorionic villi isolated from term placentae. Qualitative and quantitative HHV-8 detection were performed by PCR and real-time PCR, and HHV-8 proteins were analyzed by immunohistochemistry. Term placenta samples from HHV-8-seropositive women were analyzed for the presence of HHV-8 DNA and antigens. In vitro infected histocultures showed increasing amounts of HHV-8 DNA in tissues and supernatants; cyto- and syncitiotrophoblasts, as well as endothelial cells, expressed latent and lytic viral antigens. Increased apoptotic phenomena were visualized by the terminal deoxynucleotidyl transferase-mediated deoxyuridine nick end-labeling method in infected histocultures. Ex vivo, HHV-8 DNA and a latent viral antigen were detected in placenta samples from HHV-8-seropositive women. These findings demonstrate that HHV-8, like other human herpesviruses, may infect placental cells in vitro and in vivo, thus providing evidence that this phenomenon might influence vertical transmission and pregnancy outcome in HHV-8-infected women. Public Library of Science 2008-12-30 /pmc/articles/PMC2603597/ /pubmed/19115001 http://dx.doi.org/10.1371/journal.pone.0004073 Text en Di Stefano et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Di Stefano, Mariantonietta Calabrò, Maria Luisa Di Gangi, Iole Maria Cantatore, Santina Barbierato, Massimo Bergamo, Elisa Kfutwah, Anfumbom Jude Neri, Margherita Chieco-Bianchi, Luigi Greco, Pantaleo Gesualdo, Loreto Ayouba, Ahidjo Menu, Elisabeth Fiore, Josè Ramòn In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta |
title |
In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta |
title_full |
In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta |
title_fullStr |
In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta |
title_full_unstemmed |
In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta |
title_short |
In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta |
title_sort | in vitro and in vivo human herpesvirus 8 infection of placenta |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2603597/ https://www.ncbi.nlm.nih.gov/pubmed/19115001 http://dx.doi.org/10.1371/journal.pone.0004073 |
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