Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

BACKGROUND: HLA-A2 tetramer flow cytometry, IFNγ real time RT-PCR and IFNγ ELISPOT assays are commonly used as surrogate immunological endpoints for cancer immunotherapy. While these are often used as research assays to assess patient's immunologic response, assay validation is necessary to ens...

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Detalles Bibliográficos
Autores principales: Xu, Yuanxin, Theobald, Valerie, Sung, Crystal, DePalma, Kathleen, Atwater, Laura, Seiger, Keirsten, Perricone, Michael A, Richards, Susan M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605437/
https://www.ncbi.nlm.nih.gov/pubmed/18945350
http://dx.doi.org/10.1186/1479-5876-6-61

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