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Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity

OBJECTIVE—Osteopontin (OPN) plays an important role in the development of insulin resistance and liver complications in dietary murine models. We aimed to determine the expression pattern of OPN and its receptor CD44 in obese patients and mice according to insulin resistance and liver steatosis. RES...

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Autores principales: Bertola, Adeline, Deveaux, Vanessa, Bonnafous, Stéphanie, Rousseau, Déborah, Anty, Rodolphe, Wakkach, Abdelilah, Dahman, Moncef, Tordjman, Joan, Clément, Karine, McQuaid, Siobhán E., Frayn, Keith N., Huet, Pierre-Michel, Gugenheim, Jean, Lotersztajn, Sophie, Le Marchand-Brustel, Yannick, Tran, Albert, Gual, Philippe
Formato: Texto
Lenguaje:English
Publicado: American Diabetes Association 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2606860/
https://www.ncbi.nlm.nih.gov/pubmed/18952835
http://dx.doi.org/10.2337/db08-0400
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author Bertola, Adeline
Deveaux, Vanessa
Bonnafous, Stéphanie
Rousseau, Déborah
Anty, Rodolphe
Wakkach, Abdelilah
Dahman, Moncef
Tordjman, Joan
Clément, Karine
McQuaid, Siobhán E.
Frayn, Keith N.
Huet, Pierre-Michel
Gugenheim, Jean
Lotersztajn, Sophie
Le Marchand-Brustel, Yannick
Tran, Albert
Gual, Philippe
author_facet Bertola, Adeline
Deveaux, Vanessa
Bonnafous, Stéphanie
Rousseau, Déborah
Anty, Rodolphe
Wakkach, Abdelilah
Dahman, Moncef
Tordjman, Joan
Clément, Karine
McQuaid, Siobhán E.
Frayn, Keith N.
Huet, Pierre-Michel
Gugenheim, Jean
Lotersztajn, Sophie
Le Marchand-Brustel, Yannick
Tran, Albert
Gual, Philippe
author_sort Bertola, Adeline
collection PubMed
description OBJECTIVE—Osteopontin (OPN) plays an important role in the development of insulin resistance and liver complications in dietary murine models. We aimed to determine the expression pattern of OPN and its receptor CD44 in obese patients and mice according to insulin resistance and liver steatosis. RESEARCH DESIGN AND METHODS—OPN and CD44 expressions were studied in 52 morbidly obese patients and in mice. Cellular studies were performed in HepG2 cells. RESULTS—Hepatic OPN and CD44 expressions were strongly correlated with liver steatosis and insulin resistance in obese patients and mice. This increased OPN expression could be due to the accumulation of triglycerides, since fat loading in HepG2 promotes OPN expression. In contrast, OPN expression in adipose tissue (AT) was enhanced independently of insulin resistance and hepatic steatosis in obese patients. The elevated OPN expression in AT was paralleled with the AT macrophage infiltration, and both phenomena were reversed after weight loss. The circulating OPN level was slightly elevated in obese patients and was not related to liver steatosis. Further, AT did not appear to secrete OPN. In contrast, bariatric surgery–induced weight loss induced a strong increase in circulating OPN. CONCLUSIONS—The modestly elevated circulating OPN levels in morbidly obese patients were not related to liver steatosis and did not appear to result from adipose tissue secretion. In subcutaneous AT, expression of OPN was directly related to macrophage accumulation independently from liver complications. In contrast, hepatic OPN and CD44 expressions were related to insulin resistance and steatosis, suggesting their local implication in the progression of liver injury.
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spelling pubmed-26068602010-01-01 Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity Bertola, Adeline Deveaux, Vanessa Bonnafous, Stéphanie Rousseau, Déborah Anty, Rodolphe Wakkach, Abdelilah Dahman, Moncef Tordjman, Joan Clément, Karine McQuaid, Siobhán E. Frayn, Keith N. Huet, Pierre-Michel Gugenheim, Jean Lotersztajn, Sophie Le Marchand-Brustel, Yannick Tran, Albert Gual, Philippe Diabetes Obesity Studies OBJECTIVE—Osteopontin (OPN) plays an important role in the development of insulin resistance and liver complications in dietary murine models. We aimed to determine the expression pattern of OPN and its receptor CD44 in obese patients and mice according to insulin resistance and liver steatosis. RESEARCH DESIGN AND METHODS—OPN and CD44 expressions were studied in 52 morbidly obese patients and in mice. Cellular studies were performed in HepG2 cells. RESULTS—Hepatic OPN and CD44 expressions were strongly correlated with liver steatosis and insulin resistance in obese patients and mice. This increased OPN expression could be due to the accumulation of triglycerides, since fat loading in HepG2 promotes OPN expression. In contrast, OPN expression in adipose tissue (AT) was enhanced independently of insulin resistance and hepatic steatosis in obese patients. The elevated OPN expression in AT was paralleled with the AT macrophage infiltration, and both phenomena were reversed after weight loss. The circulating OPN level was slightly elevated in obese patients and was not related to liver steatosis. Further, AT did not appear to secrete OPN. In contrast, bariatric surgery–induced weight loss induced a strong increase in circulating OPN. CONCLUSIONS—The modestly elevated circulating OPN levels in morbidly obese patients were not related to liver steatosis and did not appear to result from adipose tissue secretion. In subcutaneous AT, expression of OPN was directly related to macrophage accumulation independently from liver complications. In contrast, hepatic OPN and CD44 expressions were related to insulin resistance and steatosis, suggesting their local implication in the progression of liver injury. American Diabetes Association 2009-01 /pmc/articles/PMC2606860/ /pubmed/18952835 http://dx.doi.org/10.2337/db08-0400 Text en Copyright © 2009, American Diabetes Association Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.
spellingShingle Obesity Studies
Bertola, Adeline
Deveaux, Vanessa
Bonnafous, Stéphanie
Rousseau, Déborah
Anty, Rodolphe
Wakkach, Abdelilah
Dahman, Moncef
Tordjman, Joan
Clément, Karine
McQuaid, Siobhán E.
Frayn, Keith N.
Huet, Pierre-Michel
Gugenheim, Jean
Lotersztajn, Sophie
Le Marchand-Brustel, Yannick
Tran, Albert
Gual, Philippe
Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
title Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
title_full Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
title_fullStr Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
title_full_unstemmed Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
title_short Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
title_sort elevated expression of osteopontin may be related to adipose tissue macrophage accumulation and liver steatosis in morbid obesity
topic Obesity Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2606860/
https://www.ncbi.nlm.nih.gov/pubmed/18952835
http://dx.doi.org/10.2337/db08-0400
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