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Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity
OBJECTIVE—Osteopontin (OPN) plays an important role in the development of insulin resistance and liver complications in dietary murine models. We aimed to determine the expression pattern of OPN and its receptor CD44 in obese patients and mice according to insulin resistance and liver steatosis. RES...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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American Diabetes Association
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2606860/ https://www.ncbi.nlm.nih.gov/pubmed/18952835 http://dx.doi.org/10.2337/db08-0400 |
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author | Bertola, Adeline Deveaux, Vanessa Bonnafous, Stéphanie Rousseau, Déborah Anty, Rodolphe Wakkach, Abdelilah Dahman, Moncef Tordjman, Joan Clément, Karine McQuaid, Siobhán E. Frayn, Keith N. Huet, Pierre-Michel Gugenheim, Jean Lotersztajn, Sophie Le Marchand-Brustel, Yannick Tran, Albert Gual, Philippe |
author_facet | Bertola, Adeline Deveaux, Vanessa Bonnafous, Stéphanie Rousseau, Déborah Anty, Rodolphe Wakkach, Abdelilah Dahman, Moncef Tordjman, Joan Clément, Karine McQuaid, Siobhán E. Frayn, Keith N. Huet, Pierre-Michel Gugenheim, Jean Lotersztajn, Sophie Le Marchand-Brustel, Yannick Tran, Albert Gual, Philippe |
author_sort | Bertola, Adeline |
collection | PubMed |
description | OBJECTIVE—Osteopontin (OPN) plays an important role in the development of insulin resistance and liver complications in dietary murine models. We aimed to determine the expression pattern of OPN and its receptor CD44 in obese patients and mice according to insulin resistance and liver steatosis. RESEARCH DESIGN AND METHODS—OPN and CD44 expressions were studied in 52 morbidly obese patients and in mice. Cellular studies were performed in HepG2 cells. RESULTS—Hepatic OPN and CD44 expressions were strongly correlated with liver steatosis and insulin resistance in obese patients and mice. This increased OPN expression could be due to the accumulation of triglycerides, since fat loading in HepG2 promotes OPN expression. In contrast, OPN expression in adipose tissue (AT) was enhanced independently of insulin resistance and hepatic steatosis in obese patients. The elevated OPN expression in AT was paralleled with the AT macrophage infiltration, and both phenomena were reversed after weight loss. The circulating OPN level was slightly elevated in obese patients and was not related to liver steatosis. Further, AT did not appear to secrete OPN. In contrast, bariatric surgery–induced weight loss induced a strong increase in circulating OPN. CONCLUSIONS—The modestly elevated circulating OPN levels in morbidly obese patients were not related to liver steatosis and did not appear to result from adipose tissue secretion. In subcutaneous AT, expression of OPN was directly related to macrophage accumulation independently from liver complications. In contrast, hepatic OPN and CD44 expressions were related to insulin resistance and steatosis, suggesting their local implication in the progression of liver injury. |
format | Text |
id | pubmed-2606860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | American Diabetes Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-26068602010-01-01 Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity Bertola, Adeline Deveaux, Vanessa Bonnafous, Stéphanie Rousseau, Déborah Anty, Rodolphe Wakkach, Abdelilah Dahman, Moncef Tordjman, Joan Clément, Karine McQuaid, Siobhán E. Frayn, Keith N. Huet, Pierre-Michel Gugenheim, Jean Lotersztajn, Sophie Le Marchand-Brustel, Yannick Tran, Albert Gual, Philippe Diabetes Obesity Studies OBJECTIVE—Osteopontin (OPN) plays an important role in the development of insulin resistance and liver complications in dietary murine models. We aimed to determine the expression pattern of OPN and its receptor CD44 in obese patients and mice according to insulin resistance and liver steatosis. RESEARCH DESIGN AND METHODS—OPN and CD44 expressions were studied in 52 morbidly obese patients and in mice. Cellular studies were performed in HepG2 cells. RESULTS—Hepatic OPN and CD44 expressions were strongly correlated with liver steatosis and insulin resistance in obese patients and mice. This increased OPN expression could be due to the accumulation of triglycerides, since fat loading in HepG2 promotes OPN expression. In contrast, OPN expression in adipose tissue (AT) was enhanced independently of insulin resistance and hepatic steatosis in obese patients. The elevated OPN expression in AT was paralleled with the AT macrophage infiltration, and both phenomena were reversed after weight loss. The circulating OPN level was slightly elevated in obese patients and was not related to liver steatosis. Further, AT did not appear to secrete OPN. In contrast, bariatric surgery–induced weight loss induced a strong increase in circulating OPN. CONCLUSIONS—The modestly elevated circulating OPN levels in morbidly obese patients were not related to liver steatosis and did not appear to result from adipose tissue secretion. In subcutaneous AT, expression of OPN was directly related to macrophage accumulation independently from liver complications. In contrast, hepatic OPN and CD44 expressions were related to insulin resistance and steatosis, suggesting their local implication in the progression of liver injury. American Diabetes Association 2009-01 /pmc/articles/PMC2606860/ /pubmed/18952835 http://dx.doi.org/10.2337/db08-0400 Text en Copyright © 2009, American Diabetes Association Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details. |
spellingShingle | Obesity Studies Bertola, Adeline Deveaux, Vanessa Bonnafous, Stéphanie Rousseau, Déborah Anty, Rodolphe Wakkach, Abdelilah Dahman, Moncef Tordjman, Joan Clément, Karine McQuaid, Siobhán E. Frayn, Keith N. Huet, Pierre-Michel Gugenheim, Jean Lotersztajn, Sophie Le Marchand-Brustel, Yannick Tran, Albert Gual, Philippe Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity |
title | Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity |
title_full | Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity |
title_fullStr | Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity |
title_full_unstemmed | Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity |
title_short | Elevated Expression of Osteopontin May Be Related to Adipose Tissue Macrophage Accumulation and Liver Steatosis in Morbid Obesity |
title_sort | elevated expression of osteopontin may be related to adipose tissue macrophage accumulation and liver steatosis in morbid obesity |
topic | Obesity Studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2606860/ https://www.ncbi.nlm.nih.gov/pubmed/18952835 http://dx.doi.org/10.2337/db08-0400 |
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