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EBs clip CLIPs to growing microtubule ends

Proteins that track growing microtubule (MT) ends are important for many aspects of intracellular MT function, but the mechanism by which these +TIPs accumulate at MT ends has been the subject of a long-standing controversy. In this issue, Bieling et al. (Bieling, P., S. Kandels-Lewis, I.A. Telley,...

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Detalles Bibliográficos
Autor principal: Wittmann, Torsten
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2606966/
https://www.ncbi.nlm.nih.gov/pubmed/19103811
http://dx.doi.org/10.1083/jcb.200811136
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author Wittmann, Torsten
author_facet Wittmann, Torsten
author_sort Wittmann, Torsten
collection PubMed
description Proteins that track growing microtubule (MT) ends are important for many aspects of intracellular MT function, but the mechanism by which these +TIPs accumulate at MT ends has been the subject of a long-standing controversy. In this issue, Bieling et al. (Bieling, P., S. Kandels-Lewis, I.A. Telley, J. van Dijk, C. Janke, and T. Surrey. 2008. J. Cell Biol. 183:1223–1233) reconstitute plus end tracking of EB1 and CLIP-170 in vitro, which demonstrates that CLIP-170 plus end tracking is EB1-dependent and that both +TIPs rapidly exchange between a soluble and a plus end–associated pool. This strongly supports the hypothesis that plus end tracking depends on a biochemical property of growing MT ends, and that the characteristic +TIP comets result from the generation of new +TIP binding sites through MT polymerization in combination with the exponential decay of these binding sites.
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spelling pubmed-26069662009-06-29 EBs clip CLIPs to growing microtubule ends Wittmann, Torsten J Cell Biol Reviews Proteins that track growing microtubule (MT) ends are important for many aspects of intracellular MT function, but the mechanism by which these +TIPs accumulate at MT ends has been the subject of a long-standing controversy. In this issue, Bieling et al. (Bieling, P., S. Kandels-Lewis, I.A. Telley, J. van Dijk, C. Janke, and T. Surrey. 2008. J. Cell Biol. 183:1223–1233) reconstitute plus end tracking of EB1 and CLIP-170 in vitro, which demonstrates that CLIP-170 plus end tracking is EB1-dependent and that both +TIPs rapidly exchange between a soluble and a plus end–associated pool. This strongly supports the hypothesis that plus end tracking depends on a biochemical property of growing MT ends, and that the characteristic +TIP comets result from the generation of new +TIP binding sites through MT polymerization in combination with the exponential decay of these binding sites. The Rockefeller University Press 2008-12-29 /pmc/articles/PMC2606966/ /pubmed/19103811 http://dx.doi.org/10.1083/jcb.200811136 Text en © 2008 Wittmann This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Reviews
Wittmann, Torsten
EBs clip CLIPs to growing microtubule ends
title EBs clip CLIPs to growing microtubule ends
title_full EBs clip CLIPs to growing microtubule ends
title_fullStr EBs clip CLIPs to growing microtubule ends
title_full_unstemmed EBs clip CLIPs to growing microtubule ends
title_short EBs clip CLIPs to growing microtubule ends
title_sort ebs clip clips to growing microtubule ends
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2606966/
https://www.ncbi.nlm.nih.gov/pubmed/19103811
http://dx.doi.org/10.1083/jcb.200811136
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