Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer

Tumour suppressor genes (TSGs) were frequently inactivated through promoter hypermethylation in gastric carcinoma as well as pre-malignant gastric lesions, suggesting that promoter hypermethylation can be used as a marker to define novel TSGs and also biomarkers for early detection of gastric cancer...

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Autores principales: Cheng, Y Y, Jin, H, Liu, X, Siu, J M T, Wong, Y P, Ng, E K O, Yu, J, Leung, W-k, Sung, J J Y, Chan, F K L
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2008
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2607230/
https://www.ncbi.nlm.nih.gov/pubmed/18985039
http://dx.doi.org/10.1038/sj.bjc.6604760
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author Cheng, Y Y
Jin, H
Liu, X
Siu, J M T
Wong, Y P
Ng, E K O
Yu, J
Leung, W-k
Sung, J J Y
Chan, F K L
author_facet Cheng, Y Y
Jin, H
Liu, X
Siu, J M T
Wong, Y P
Ng, E K O
Yu, J
Leung, W-k
Sung, J J Y
Chan, F K L
author_sort Cheng, Y Y
collection PubMed
description Tumour suppressor genes (TSGs) were frequently inactivated through promoter hypermethylation in gastric carcinoma as well as pre-malignant gastric lesions, suggesting that promoter hypermethylation can be used as a marker to define novel TSGs and also biomarkers for early detection of gastric cancer. In an effort to search for such genes aberrantly methylated in gastric cancer development, fibulin 1 (FBLN1) was found as a candidate TSG epigenetically downregulated in gastric cancer. FBLN1 expression was downregulated in all of gastric cancer cell lines used (100%, 7 out of 7) and the primary gastric carcinoma tissues (84%, 86 out of 102) and significantly restored after pharmacological demethylation. Hypermethylation of the FBLN1 promoter was frequently (71%, 5 out of 7) detected in gastric cancer cell lines and primary gastric carcinoma tissues. Ectopic expression of FBLN1 led to the growth inhibition of gastric cancer cells through the induction of apoptosis. In summary, FBLN1 was identified as a novel candidate TSG epigenetically downregulated in gastric cancer.
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spelling pubmed-26072302009-12-09 Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer Cheng, Y Y Jin, H Liu, X Siu, J M T Wong, Y P Ng, E K O Yu, J Leung, W-k Sung, J J Y Chan, F K L Br J Cancer Genetics and Genomics Tumour suppressor genes (TSGs) were frequently inactivated through promoter hypermethylation in gastric carcinoma as well as pre-malignant gastric lesions, suggesting that promoter hypermethylation can be used as a marker to define novel TSGs and also biomarkers for early detection of gastric cancer. In an effort to search for such genes aberrantly methylated in gastric cancer development, fibulin 1 (FBLN1) was found as a candidate TSG epigenetically downregulated in gastric cancer. FBLN1 expression was downregulated in all of gastric cancer cell lines used (100%, 7 out of 7) and the primary gastric carcinoma tissues (84%, 86 out of 102) and significantly restored after pharmacological demethylation. Hypermethylation of the FBLN1 promoter was frequently (71%, 5 out of 7) detected in gastric cancer cell lines and primary gastric carcinoma tissues. Ectopic expression of FBLN1 led to the growth inhibition of gastric cancer cells through the induction of apoptosis. In summary, FBLN1 was identified as a novel candidate TSG epigenetically downregulated in gastric cancer. Nature Publishing Group 2008-12-09 2008-11-04 /pmc/articles/PMC2607230/ /pubmed/18985039 http://dx.doi.org/10.1038/sj.bjc.6604760 Text en Copyright © 2008 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Genetics and Genomics
Cheng, Y Y
Jin, H
Liu, X
Siu, J M T
Wong, Y P
Ng, E K O
Yu, J
Leung, W-k
Sung, J J Y
Chan, F K L
Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer
title Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer
title_full Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer
title_fullStr Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer
title_full_unstemmed Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer
title_short Fibulin 1 is downregulated through promoter hypermethylation in gastric cancer
title_sort fibulin 1 is downregulated through promoter hypermethylation in gastric cancer
topic Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2607230/
https://www.ncbi.nlm.nih.gov/pubmed/18985039
http://dx.doi.org/10.1038/sj.bjc.6604760
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