Cargando…

Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature

In malignant gliomas, the integrin adhesion receptors seem to play a key role for invasive growth and angiogenesis. However, there is still a controversy about the expression and the distribution of α(v)β(3) integrin caused by malignancy. The aim of our study was to assess the extent and pattern of...

Descripción completa

Detalles Bibliográficos
Autores principales: Schnell, Oliver, Krebs, Bjarne, Wagner, Erika, Romagna, Alexander, Beer, Ambros J., Grau, Stefan J., Thon, Niklas, Goetz, Claudia, Kretzschmar, Hans A., Tonn, Jörg‐Christian, Goldbrunner, Roland H.
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2607528/
https://www.ncbi.nlm.nih.gov/pubmed/18394009
http://dx.doi.org/10.1111/j.1750-3639.2008.00137.x
_version_ 1782163051845255168
author Schnell, Oliver
Krebs, Bjarne
Wagner, Erika
Romagna, Alexander
Beer, Ambros J.
Grau, Stefan J.
Thon, Niklas
Goetz, Claudia
Kretzschmar, Hans A.
Tonn, Jörg‐Christian
Goldbrunner, Roland H.
author_facet Schnell, Oliver
Krebs, Bjarne
Wagner, Erika
Romagna, Alexander
Beer, Ambros J.
Grau, Stefan J.
Thon, Niklas
Goetz, Claudia
Kretzschmar, Hans A.
Tonn, Jörg‐Christian
Goldbrunner, Roland H.
author_sort Schnell, Oliver
collection PubMed
description In malignant gliomas, the integrin adhesion receptors seem to play a key role for invasive growth and angiogenesis. However, there is still a controversy about the expression and the distribution of α(v)β(3) integrin caused by malignancy. The aim of our study was to assess the extent and pattern of α(v)β(3) integrin expression within primary glioblastomas (GBMs) compared with low‐grade gliomas (LGGs). Tumor samples were immunostained for the detection of α(v)β(3) integrin and quantified by an imaging software. The expression of α(v)β(3) was found to be significantly higher in GBMs than in LGGs, whereby focal strong reactivity was restricted to GBMs only. Subsequent analysis revealed that not only endothelial cells but also, to a large extent, glial tumor cells contribute to the overall amount of α(v)β(3) integrin in the tumors. To further analyze the integrin subunits, Western blots from histologic sections were performed, which demonstrated a significant difference in the expression of the β(3) integrin subunit between GBMs and LGGs. The presented data lead to new insights in the pattern of α(v)β(3) integrin in gliomas and are of relevance for the inhibition of α(v)β(3) integrin with specific RGD peptides and interfering drugs to reduce angiogenesis and tumor growth.
format Text
id pubmed-2607528
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-26075282008-12-29 Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature Schnell, Oliver Krebs, Bjarne Wagner, Erika Romagna, Alexander Beer, Ambros J. Grau, Stefan J. Thon, Niklas Goetz, Claudia Kretzschmar, Hans A. Tonn, Jörg‐Christian Goldbrunner, Roland H. Brain Pathol Research Articles In malignant gliomas, the integrin adhesion receptors seem to play a key role for invasive growth and angiogenesis. However, there is still a controversy about the expression and the distribution of α(v)β(3) integrin caused by malignancy. The aim of our study was to assess the extent and pattern of α(v)β(3) integrin expression within primary glioblastomas (GBMs) compared with low‐grade gliomas (LGGs). Tumor samples were immunostained for the detection of α(v)β(3) integrin and quantified by an imaging software. The expression of α(v)β(3) was found to be significantly higher in GBMs than in LGGs, whereby focal strong reactivity was restricted to GBMs only. Subsequent analysis revealed that not only endothelial cells but also, to a large extent, glial tumor cells contribute to the overall amount of α(v)β(3) integrin in the tumors. To further analyze the integrin subunits, Western blots from histologic sections were performed, which demonstrated a significant difference in the expression of the β(3) integrin subunit between GBMs and LGGs. The presented data lead to new insights in the pattern of α(v)β(3) integrin in gliomas and are of relevance for the inhibition of α(v)β(3) integrin with specific RGD peptides and interfering drugs to reduce angiogenesis and tumor growth. Blackwell Publishing Ltd 2008-04-03 /pmc/articles/PMC2607528/ /pubmed/18394009 http://dx.doi.org/10.1111/j.1750-3639.2008.00137.x Text en © 2008 The Authors. Journal Compilation © 2008 International Society of Neuropathology Open access.
spellingShingle Research Articles
Schnell, Oliver
Krebs, Bjarne
Wagner, Erika
Romagna, Alexander
Beer, Ambros J.
Grau, Stefan J.
Thon, Niklas
Goetz, Claudia
Kretzschmar, Hans A.
Tonn, Jörg‐Christian
Goldbrunner, Roland H.
Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature
title Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature
title_full Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature
title_fullStr Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature
title_full_unstemmed Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature
title_short Expression of Integrin α(v)β(3) in Gliomas Correlates with Tumor Grade and Is not Restricted to Tumor Vasculature
title_sort expression of integrin α(v)β(3) in gliomas correlates with tumor grade and is not restricted to tumor vasculature
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2607528/
https://www.ncbi.nlm.nih.gov/pubmed/18394009
http://dx.doi.org/10.1111/j.1750-3639.2008.00137.x
work_keys_str_mv AT schnelloliver expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT krebsbjarne expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT wagnererika expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT romagnaalexander expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT beerambrosj expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT graustefanj expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT thonniklas expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT goetzclaudia expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT kretzschmarhansa expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT tonnjorgchristian expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature
AT goldbrunnerrolandh expressionofintegrinavb3ingliomascorrelateswithtumorgradeandisnotrestrictedtotumorvasculature