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Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs
BACKGROUND: An ideal format to describe transcriptome would be its composition measured on the scale of absolute numbers of individual mRNAs per cell. It would help not only to precisely grasp the structure of the transcriptome but also to accelerate data exchange and integration. RESULTS: We concei...
Autores principales: | Miura, Fumihito, Kawaguchi, Noriko, Yoshida, Mikio, Uematsu, Chihiro, Kito, Keiji, Sakaki, Yoshiyuki, Ito, Takashi |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2612024/ https://www.ncbi.nlm.nih.gov/pubmed/19040753 http://dx.doi.org/10.1186/1471-2164-9-574 |
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