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Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans
When Caenorhabditis elegans senses dauer pheromone (daumone), signaling inadequate growth conditions, it enters the dauer state, which is capable of long-term survival. However, the molecular pathway of dauer entry in C. elegans has remained elusive. To systematically monitor changes in gene express...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2612749/ https://www.ncbi.nlm.nih.gov/pubmed/19129915 http://dx.doi.org/10.1371/journal.pone.0004162 |
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author | Jeong, Pan-Young Kwon, Min-Seok Joo, Hyoe-Jin Paik, Young-Ki |
author_facet | Jeong, Pan-Young Kwon, Min-Seok Joo, Hyoe-Jin Paik, Young-Ki |
author_sort | Jeong, Pan-Young |
collection | PubMed |
description | When Caenorhabditis elegans senses dauer pheromone (daumone), signaling inadequate growth conditions, it enters the dauer state, which is capable of long-term survival. However, the molecular pathway of dauer entry in C. elegans has remained elusive. To systematically monitor changes in gene expression in dauer paths, we used a DNA microarray containing 22,625 gene probes corresponding to 22,150 unique genes from C. elegans. We employed two different paths: direct exposure to daumone (Path 1) and normal growth media plus liquid culture (Path 2). Our data reveal that entry into dauer is accomplished through the multi-step process, which appears to be compartmentalized in time and according to metabolic flux. That is, a time-course of dauer entry in Path 1 shows that dauer larvae formation begins at post-embryonic stage S4 (48 h) and is complete at S6 (72 h). Our results also suggest the presence of a unique adaptive metabolic control mechanism that requires both stage-specific expression of specific genes and tight regulation of different modes of fuel metabolite utilization to sustain the energy balance in the context of prolonged survival under adverse growth conditions. It is apparent that worms entering dauer stage may rely heavily on carbohydrate-based energy reserves, whereas dauer larvae utilize fat or glyoxylate cycle-based energy sources. We created a comprehensive web-based dauer metabolic database for C. elegans (www.DauerDB.org) that makes it possible to search any gene and compare its relative expression at a specific stage, or evaluate overall patterns of gene expression in both paths. This database can be accessed by the research community and could be widely applicable to other related nematodes as a molecular atlas. |
format | Text |
id | pubmed-2612749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26127492009-01-08 Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans Jeong, Pan-Young Kwon, Min-Seok Joo, Hyoe-Jin Paik, Young-Ki PLoS One Research Article When Caenorhabditis elegans senses dauer pheromone (daumone), signaling inadequate growth conditions, it enters the dauer state, which is capable of long-term survival. However, the molecular pathway of dauer entry in C. elegans has remained elusive. To systematically monitor changes in gene expression in dauer paths, we used a DNA microarray containing 22,625 gene probes corresponding to 22,150 unique genes from C. elegans. We employed two different paths: direct exposure to daumone (Path 1) and normal growth media plus liquid culture (Path 2). Our data reveal that entry into dauer is accomplished through the multi-step process, which appears to be compartmentalized in time and according to metabolic flux. That is, a time-course of dauer entry in Path 1 shows that dauer larvae formation begins at post-embryonic stage S4 (48 h) and is complete at S6 (72 h). Our results also suggest the presence of a unique adaptive metabolic control mechanism that requires both stage-specific expression of specific genes and tight regulation of different modes of fuel metabolite utilization to sustain the energy balance in the context of prolonged survival under adverse growth conditions. It is apparent that worms entering dauer stage may rely heavily on carbohydrate-based energy reserves, whereas dauer larvae utilize fat or glyoxylate cycle-based energy sources. We created a comprehensive web-based dauer metabolic database for C. elegans (www.DauerDB.org) that makes it possible to search any gene and compare its relative expression at a specific stage, or evaluate overall patterns of gene expression in both paths. This database can be accessed by the research community and could be widely applicable to other related nematodes as a molecular atlas. Public Library of Science 2009-01-08 /pmc/articles/PMC2612749/ /pubmed/19129915 http://dx.doi.org/10.1371/journal.pone.0004162 Text en Jeong et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jeong, Pan-Young Kwon, Min-Seok Joo, Hyoe-Jin Paik, Young-Ki Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans |
title | Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans
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title_full | Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans
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title_fullStr | Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans
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title_full_unstemmed | Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans
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title_short | Molecular Time-Course and the Metabolic Basis of Entry into Dauer in Caenorhabditis elegans
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title_sort | molecular time-course and the metabolic basis of entry into dauer in caenorhabditis elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2612749/ https://www.ncbi.nlm.nih.gov/pubmed/19129915 http://dx.doi.org/10.1371/journal.pone.0004162 |
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