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In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1
Ubiquinol-10 (QH(2)), the reduced form of Coenzyme Q(10) (CoQ(10)) serves as a potent antioxidant of lipid membranes. Because many antioxidants reveal potent anti-inflammatory effects, the influence of QH(2) on lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and chemokines were determine...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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the Society for Free Radical Research Japan
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613501/ https://www.ncbi.nlm.nih.gov/pubmed/19177190 http://dx.doi.org/10.3164/jcbn.08-182 |
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author | Schmelzer, Constance Lorenz, Gerti Rimbach, Gerald Döring, Frank |
author_facet | Schmelzer, Constance Lorenz, Gerti Rimbach, Gerald Döring, Frank |
author_sort | Schmelzer, Constance |
collection | PubMed |
description | Ubiquinol-10 (QH(2)), the reduced form of Coenzyme Q(10) (CoQ(10)) serves as a potent antioxidant of lipid membranes. Because many antioxidants reveal potent anti-inflammatory effects, the influence of QH(2) on lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and chemokines were determined in the human monocytic cell line THP-1. Stimulation of cells with LPS resulted in a distinct release of Tumour necrosis factor-alpha (TNF-α), Macrophage inflammatory protein-1 alpha (MIP-1α), Regulated upon activation, normal T cell expressed and secreted (RANTES) and Monocyte chemotattractant protein-1 (MCP-1). The LPS-induced responses were significantly decreased by pre-incubation of cells with QH(2) to 60.27 ± 9.3% (p = 0.0009), 48.13 ± 6.93% (p = 0.0007) and 74.36 ± 7.25% (p = 0.008) for TNF-α, MIP-1α and RANTES, respectively. In conclusion, our results indicate anti-inflammatory effects of the reduced form of CoQ(10) on various proinflammatory cytokines and chemokines in vitro. |
format | Text |
id | pubmed-2613501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | the Society for Free Radical Research Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-26135012009-01-28 In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1 Schmelzer, Constance Lorenz, Gerti Rimbach, Gerald Döring, Frank J Clin Biochem Nutr Original Article Ubiquinol-10 (QH(2)), the reduced form of Coenzyme Q(10) (CoQ(10)) serves as a potent antioxidant of lipid membranes. Because many antioxidants reveal potent anti-inflammatory effects, the influence of QH(2) on lipopolysaccharide (LPS)-induced pro-inflammatory cytokines and chemokines were determined in the human monocytic cell line THP-1. Stimulation of cells with LPS resulted in a distinct release of Tumour necrosis factor-alpha (TNF-α), Macrophage inflammatory protein-1 alpha (MIP-1α), Regulated upon activation, normal T cell expressed and secreted (RANTES) and Monocyte chemotattractant protein-1 (MCP-1). The LPS-induced responses were significantly decreased by pre-incubation of cells with QH(2) to 60.27 ± 9.3% (p = 0.0009), 48.13 ± 6.93% (p = 0.0007) and 74.36 ± 7.25% (p = 0.008) for TNF-α, MIP-1α and RANTES, respectively. In conclusion, our results indicate anti-inflammatory effects of the reduced form of CoQ(10) on various proinflammatory cytokines and chemokines in vitro. the Society for Free Radical Research Japan 2009-01 2008-12-27 /pmc/articles/PMC2613501/ /pubmed/19177190 http://dx.doi.org/10.3164/jcbn.08-182 Text en Copyright © 2009 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Schmelzer, Constance Lorenz, Gerti Rimbach, Gerald Döring, Frank In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1 |
title | In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1 |
title_full | In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1 |
title_fullStr | In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1 |
title_full_unstemmed | In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1 |
title_short | In Vitro Effects of the Reduced Form of Coenzyme Q(10) on Secretion Levels of TNF-α and Chemokines in Response to LPS in the Human Monocytic Cell Line THP-1 |
title_sort | in vitro effects of the reduced form of coenzyme q(10) on secretion levels of tnf-α and chemokines in response to lps in the human monocytic cell line thp-1 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613501/ https://www.ncbi.nlm.nih.gov/pubmed/19177190 http://dx.doi.org/10.3164/jcbn.08-182 |
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