Cargando…

Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens

Lignin is incorporated into plant cell walls to maintain plant architecture and to ensure long-distance water transport. Lignin composition affects the industrial value of plant material for forage, wood and paper production, and biofuel technologies. Industrial demands have resulted in an increase...

Descripción completa

Detalles Bibliográficos
Autores principales: Quentin, Michaël, Allasia, Valérie, Pegard, Anthony, Allais, Florent, Ducrot, Paul-Henri, Favery, Bruno, Levis, Caroline, Martinet, Sophie, Masur, Clarissa, Ponchet, Michel, Roby, Dominique, Schlaich, Nikolaus L., Jouanin, Lise, Keller, Harald
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613516/
https://www.ncbi.nlm.nih.gov/pubmed/19148278
http://dx.doi.org/10.1371/journal.ppat.1000264
_version_ 1782163187896942592
author Quentin, Michaël
Allasia, Valérie
Pegard, Anthony
Allais, Florent
Ducrot, Paul-Henri
Favery, Bruno
Levis, Caroline
Martinet, Sophie
Masur, Clarissa
Ponchet, Michel
Roby, Dominique
Schlaich, Nikolaus L.
Jouanin, Lise
Keller, Harald
author_facet Quentin, Michaël
Allasia, Valérie
Pegard, Anthony
Allais, Florent
Ducrot, Paul-Henri
Favery, Bruno
Levis, Caroline
Martinet, Sophie
Masur, Clarissa
Ponchet, Michel
Roby, Dominique
Schlaich, Nikolaus L.
Jouanin, Lise
Keller, Harald
author_sort Quentin, Michaël
collection PubMed
description Lignin is incorporated into plant cell walls to maintain plant architecture and to ensure long-distance water transport. Lignin composition affects the industrial value of plant material for forage, wood and paper production, and biofuel technologies. Industrial demands have resulted in an increase in the use of genetic engineering to modify lignified plant cell wall composition. However, the interaction of the resulting plants with the environment must be analyzed carefully to ensure that there are no undesirable side effects of lignin modification. We show here that Arabidopsis thaliana mutants with impaired 5-hydroxyguaiacyl O-methyltransferase (known as caffeate O-methyltransferase; COMT) function were more susceptible to various bacterial and fungal pathogens. Unexpectedly, asexual sporulation of the downy mildew pathogen, Hyaloperonospora arabidopsidis, was impaired on these mutants. Enhanced resistance to downy mildew was not correlated with increased plant defense responses in comt1 mutants but coincided with a higher frequency of oomycete sexual reproduction within mutant tissues. Comt1 mutants but not wild-type Arabidopsis accumulated soluble 2-O-5-hydroxyferuloyl-l-malate. The compound weakened mycelium vigor and promoted sexual oomycete reproduction when applied to a homothallic oomycete in vitro. These findings suggested that the accumulation of 2-O-5-hydroxyferuloyl-l-malate accounted for the observed comt1 mutant phenotypes during the interaction with H. arabidopsidis. Taken together, our study shows that an artificial downregulation of COMT can drastically alter the interaction of a plant with the biotic environment.
format Text
id pubmed-2613516
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-26135162009-01-16 Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens Quentin, Michaël Allasia, Valérie Pegard, Anthony Allais, Florent Ducrot, Paul-Henri Favery, Bruno Levis, Caroline Martinet, Sophie Masur, Clarissa Ponchet, Michel Roby, Dominique Schlaich, Nikolaus L. Jouanin, Lise Keller, Harald PLoS Pathog Research Article Lignin is incorporated into plant cell walls to maintain plant architecture and to ensure long-distance water transport. Lignin composition affects the industrial value of plant material for forage, wood and paper production, and biofuel technologies. Industrial demands have resulted in an increase in the use of genetic engineering to modify lignified plant cell wall composition. However, the interaction of the resulting plants with the environment must be analyzed carefully to ensure that there are no undesirable side effects of lignin modification. We show here that Arabidopsis thaliana mutants with impaired 5-hydroxyguaiacyl O-methyltransferase (known as caffeate O-methyltransferase; COMT) function were more susceptible to various bacterial and fungal pathogens. Unexpectedly, asexual sporulation of the downy mildew pathogen, Hyaloperonospora arabidopsidis, was impaired on these mutants. Enhanced resistance to downy mildew was not correlated with increased plant defense responses in comt1 mutants but coincided with a higher frequency of oomycete sexual reproduction within mutant tissues. Comt1 mutants but not wild-type Arabidopsis accumulated soluble 2-O-5-hydroxyferuloyl-l-malate. The compound weakened mycelium vigor and promoted sexual oomycete reproduction when applied to a homothallic oomycete in vitro. These findings suggested that the accumulation of 2-O-5-hydroxyferuloyl-l-malate accounted for the observed comt1 mutant phenotypes during the interaction with H. arabidopsidis. Taken together, our study shows that an artificial downregulation of COMT can drastically alter the interaction of a plant with the biotic environment. Public Library of Science 2009-01-16 /pmc/articles/PMC2613516/ /pubmed/19148278 http://dx.doi.org/10.1371/journal.ppat.1000264 Text en Quentin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Quentin, Michaël
Allasia, Valérie
Pegard, Anthony
Allais, Florent
Ducrot, Paul-Henri
Favery, Bruno
Levis, Caroline
Martinet, Sophie
Masur, Clarissa
Ponchet, Michel
Roby, Dominique
Schlaich, Nikolaus L.
Jouanin, Lise
Keller, Harald
Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens
title Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens
title_full Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens
title_fullStr Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens
title_full_unstemmed Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens
title_short Imbalanced Lignin Biosynthesis Promotes the Sexual Reproduction of Homothallic Oomycete Pathogens
title_sort imbalanced lignin biosynthesis promotes the sexual reproduction of homothallic oomycete pathogens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613516/
https://www.ncbi.nlm.nih.gov/pubmed/19148278
http://dx.doi.org/10.1371/journal.ppat.1000264
work_keys_str_mv AT quentinmichael imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT allasiavalerie imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT pegardanthony imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT allaisflorent imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT ducrotpaulhenri imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT faverybruno imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT leviscaroline imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT martinetsophie imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT masurclarissa imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT ponchetmichel imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT robydominique imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT schlaichnikolausl imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT jouaninlise imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens
AT kellerharald imbalancedligninbiosynthesispromotesthesexualreproductionofhomothallicoomycetepathogens